SEEDLING LENGTH AND DETERMINATION OF VIGOR INDEXES IN SOYBEAN SEEDS THROUGH IMAGE ANALYSIS
DOI:
https://doi.org/10.32404/rean.v13i1.9745Keywords:
Glycine max, Seed vigor, Seedling performance, Computerized image analysisAbstract
Tests based on seedling performance, such as length measurements, require skilled labor and may become time-consuming depending on the number of seed lots analyzed. Computerized image analysis offers an alternative for quickly and efficiently evaluating the physiological potential of seeds, although details such as seed positioning can influence the results. This study aimed to verify the effectiveness of image analysis and to determine vigor and seedling growth indexes in soybeans based on seed positioning in the seedling length test. Seeds from four cultivars were used to assess quality through water content, germination, electrical conductivity, seedling length and dry mass tests, with BMX DESAFIO cultivar with three vigor levels (high, medium, and low) being used for the image analysis. In the seedling length test, seeds were placed on the substrate with the hilum oriented: (1) to one of the paper's sides, (2) upward, and (3) downward. The experimental design was a completely randomized 3x3 factorial scheme (vigor levels x seed positions). Measurements included hypocotyl length, root length, seedling vigor, growth, uniformity, germination, average length, and dry mass. The position and vigor of soybean seeds influence seedling growth, which was verified through the Vigor-S system. The Vigor-S system is effective for evaluating seedling length in relation to seed vigor and positioning. Positioning the hilum of medium- and high-vigor soybean seeds to the side of the paper is also a viable alternative for seedling length tests.
References
(I) Bewley, J.D., Bradford, K.J., Hilhorst, H.W.M., Nonogaki, H., 2013. Seeds: physiology of development, germination and dormancy. 3rd edition. Seed Sci. Res., 23(4), 392 pp. https://doi.org/10.1007/978-1-4614-4693-4
(II) BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Regras para Análise de Sementes. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Coordenação-Geral de Laboratórios Agropecuários – CGAL. Brasília: MAPA, 2025. https://wikisda.agricultura.gov.br/pt-br/Laborat%C3%B3rios/Metodologia/Sementes/RAS_2024
(III) BRASIL. Ministério da Agricultura, Pecuária e Abastecimento, 2013. Instrução Normativa no 45, de 17 de setembro de 2013. Padrões para a produção e a comercialização de sementes. Diário Oficial da Uniao.
(IV) Cardoso, E.A., Alves, E.U., Bruno, R.L.A., Alves, A.U., Silva, K.B., 2008. Emergência de plântulas de Erythrina velutina em diferentes posições e profundidades de semeadura. Ciência Rural, 38(9), 26182621. https://doi.org/10.1590/S0103-84782008000900034
(V) Castan, D.O.C., Gomes-Junior, F.G., Marcos-Filho, J., 2018. Vigor-S, a new system for evaluating the physiological potential of maize seeds. Scientia Agricola, 75(2), 167172. http://dx.doi.org/10.1590/1678-992x-2016-0401
(VI) Catão, H.C.R.M., Pontes, B.S., Pinheiro, D.T., Oliveira Filho, M.A., Santos, A.L.C., Zolla, M.C., 2024. Chemical treatment and mobilization of reserves of soybean seeds under water deficit. Journal of Seed Science, 46, e202446005. https://doi.org/10.1590/2317-1545v46278828
(VII) CONAB. Companhia Nacional de Abastecimento, 2026. Acompanhamento de safra brasileira: grãos, décimo segundo levantamento, junho 2026. Brasília: CONAB, 2026. Disponível em: https://www.conab.gov.br/info-agro/safras/graos/boletim-da-safra-de-graos.
(VIII) Finch-Savage, W.E., 2020. Influence of seed quality on crop establishment, growth, and yield. In: Seed quality. CRC Press, p. 361-384. https://doi.org/10.4324/9781003075226-11
(IX) Gomes-Junior, F.G. Análise computadorizada de imagens de plântulas. In: Krzyzanowski, F.C.; Vieira, R.D.; França-Neto, J.B.; Marcos-Filho, J. (Eds.). Vigor de sementes: conceitos e testes Londrina: Abrates, 2020. p.141-183
(X) Gomes-Junior, F.G., Chamma, H.M.C.P., Cicero, S.M., 2014. Automated image analysis of seedlings for vigor evaluation of common bean seeds. Acta Scientiarum. Agronomy, 36(2), 195200. https://doi.org/10.4025/actasciagron.v36i2.21957
(XI) Guedes, R.S., Alves, E.U., Santos-Moura, S.S., Galindo, E.A., 2015. Teste de comprimento de plântula na avaliação da qualidade fisiológica de sementes de Amburana cearensis. Semina: Ciências Agrarias, 36(4), 23732382. https://doi.org/10.5433/1679-0359.2015v36n4p2373
(XII) Henning, F.A., Mertz, L.M., Jacob Junior, E.A., Machado, R.D., Fiss, G., Zimmer, P.D., 2010. Composição química e mobilização de reservas em sementes de soja de alto e baixo vigor. Bragantia, 69(3), 727734. https://doi.org/10.1590/S0006-87052010000300026
(XIII) Krzyzanowski, F.C., Franca-Neto, J.B., Gomes-Junior, F.G., Nakagawa, J., 2020. Testes de vigor baseados em desempenho de plântulas. In: Krzyzanowski, F.C., Vieira, R.D., França-Neto, J.B., Marcos-Filho, J. (Eds.) Vigor de sementes: conceitos e testes. Londrina: Abrates, p.79-140.
(XIV) Leite, C.A.M., França-Neto, J.B., Krzyzanowski, F.C., Gomes-Junior, F.G., Lopes, I.O.N., Henning, F.A., 2024. Validation of the Vigor-S image analysis system for the characterization of phytotoxicities in soybean seedlings. Journal of Seed Science, 46, e202446014. https://doi.org/10.1590/2317-1545v46279386
(XV) Leite, M.S., Torres, S.B., Gomes Junior, F.G., Rego, C.H.Q., Paiva, E.P., Leite, T.S., 2020. Viability of seedling image analysis (Vigor-S) to determine the physiological potential of melon seeds. Journal of Seed Science, 42, e202042043. https://doi.org/10.1590/2317-1545v42237826
(XVI) Marcos-Filho, J., 2015a. Fisiologia de sementes de plantas cultivadas. FEALQ, Piracicaba, 495p.
(XVII) Marcos-Filho, J., 2015b. Seed vigor testing: an overview of the past, present and future perspective. Scientia Agricola, 72(4), 363374. https://doi.org/10.1590/0103-9016-2015-0007
(XVIII) Marcos-Filho, J., 2020. Testes de vigor: importância e utilização. In: Krzyzanowski, F.C., Vieira, R.D., França-Neto, J.B., Marcos-Filho, J. (Eds.) Vigor de sementes: conceitos e testes. Londrina: Abrates, p. 17-72.
(XIX) Marcos-Filho, J., Kikuti, A.L.P., Lima, L.B., 2009. Métodos para avaliação do vigor de sementes de soja, incluindo a análise computadorizada de imagens. Revista Brasileira De Sementes, 31(1), 102112. https://doi.org/10.1590/S0101-31222009000100012
(XX) Masilamani, P., Venkatesan, S., Navamaniraj, K.N., Rajarathinam, P., Alagesan, A., Thiagu, K., 2023. Impact of the orientation of seed placement and depth of its sowing on germination: a review. Journal of Applied and Natural Science, 15(1), 314324. https://doi.org/10.31018/jans.v15i1.4265
(XXI) Medeiros, A.D.D., Silva, L.J.D., Capobiango, N.P., Fialho, C.A., Dias, D.C.F.D.S., 2019. Assessing the physiological quality of common bean seeds using the Vigor-S system. Journal of Seed Science, 41(2), 187195. https://doi.org/10.1590/2317-1545v41n2211401
(XXII) Nerling, D., Coelho, C.M.M., Brummer, A., 2022. The hydrolysis dynamic of storage reserves in maize seed germination. Journal of Seed Science, 44, e202244019. https://doi.org/10.1590/2317-1545v44258701
(XXIII) Padilha, M.S., Coelho, C.M.M., Siega, Y.P., Ehrhardt-Brocardo, N.C.M., 2024. Vigor and reserve mobilization of common bean seed during germination under salt stress. Bragantia, 84, e20240079. https://doi.org/10.1590/1678-4499.20240079
(XXIV) Pinheiro, D.T., Dias, D.C.F.S., Silva, L.J.D., Martins, M.S., Finger, F.L., 2023. Oxidative stress, protein, and physiological potential of soybean seeds under weathering deterioration. Acta Scientiarum. Agronomy, 45, e56910. https://doi.org/10.4025/actasciagron.v45i1.56910
(XXV) R Core Team, 2022. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/
(XXVI) Reed, R.C., Bradford, K.J., Khanday, I., 2022. Seed germination and vigor: ensuring crop sustainability in a changing climate. Heredity, 128(6), 450-459. https://doi.org/10.1038/s41437-022-00497-2
(XXVII) Rego, C.H.Q., Cicero, S.M., França-Silva, F., Gomes-Junior, F.G., 2021. Assessing the vigor of cowpea seeds using the Vigor-S software. Journal of Seed Science, 43, e202143018. https://doi.org/10.1590/2317-1545v43244858
(XXVIII) Rodrigues, M., Gomes-Junior, F.G., Marcos-Filho, J., 2020. Vigor-S: System for Automated Analysis of Soybean Seed Vigor. Journal of Seed Science, 42, e202042039. https://doi.org/10.1590/2317-1545v42237490
(XXIX) Vieira, R.D., Bittencourt, S.R.M., Panobianco, M., 2003. Seed vigour - an important component of seed quality in Brazil. ISTA - Seed Testing International, 126, 2122.
(XXX) Vieira, R.D., Marcos-Filho, J. 2020. Teste de condutividade elétrica. In: Krzyzanowski, F.C., Vieira, R.D., França-Neto, J.B., Marcos-Filho, J. (Eds.) Vigor de sementes: conceitos e testes. Londrina: Abrates, p.93-97.
(XXXI) Wendt, L., Gomes-Junior, F.G., Zorato, M.F., Moreira, G.C., 2014. Avaliação do potencial fisiológico de sementes de soja por meio de imagens. Pesquisa Agropecuária Tropical, 44(3), 280286. https://doi.org/10.1590/S1983-40632014000300011
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