SYNERGISTIC EFFECTS OF MICROBIAL INOCULANTS AND REDUCED NUTRIENTS ON HYDROPONIC LETTUCE PERFORMANCE AND PROFITABILITY

Authors

DOI:

https://doi.org/10.32404/rean.v13i1.9766

Keywords:

Azospirillum brasilense, VAM, Nutrient solution, Lactuca sativa

Abstract

This study, conducted in Maguindanao, Philippines, addresses the high costs and environmental impacts of chemical fertilizers in hydroponics by investigating the integration of microbial inoculants with reduced-rate nutrient solutions for loose-leaf lettuce cultivation. The research used a factorial design, assessing three nutrient levels (100% recommended rate, 50%, and tap water) and four microbial conditions: no inoculant, Azospirillum brasilense, Vesicular Arbuscular Mycorrhizae (VAM), and a combination of both. A strong synergistic interaction was discovered. The treatment combining a 50% nutrient solution with both A. brasilense and VAM yielded the highest marketable biomass (446.67 g/plant), significantly surpassing the standard 100% nutrient treatment (372.51 g). Conversely, adding inoculants to the full-strength nutrient solution often suppressed plant growth. Economic analysis confirmed that the synergistic 50% nutrient and combined inoculant strategy also generated the highest annual net income, making it the most commercially viable approach. These findings demonstrate that integrating multi-strain microbial inoculants with a half-strength nutrient solution is an economically advantageous and sustainable strategy to reduce fertilizer dependency, lower production costs, and enhance hydroponic lettuce cultivation.

Author Biographies

Marlon Garrigues, North Eastern Mindanao State University

North Eastern Mindanao State University, San Miguel Campus, San Miguel, Surigao del Sur, Philippines.

Richel Barlisan Garrigues, North Eastern Mindanao State University

North Eastern Mindanao State University, San Miguel Campus, San Miguel, Surigao del Sur, Philippines.

References

(I) Aini, N., Yamika, W.S.D., Ulum, B., 2019. Effect of nutrient concentration, PGPR and AMF on plant growth, yield, and nutrient uptake of hydroponic lettuce. International Journal Of Agriculture & Biology., 21:175-183. 10.17957/IJAB/15.0879

(II) Cassán, F., Coniglio, A., López, G., Molina, R., Nievas, S., Carlan, C.N., Donadio, F., Torres D., Rosas, S., Pedrosa F.O., Souza, E., Zorita, M.D., de-Vashan, L., Mora, V., 2020. Everything you must know about Azospirillum and its impact on agriculture and beyond. Biology and Fertility of Soils, 56(4), 461-479. 10.1007/s00374-020-01463-y

(III) Dasgan, H.Y.,Temtek, T., 2022. Impact of biofertilizers on plant growth, physiological and quality traits of lettuce (Lactuca sativa L. var. Longifolia) grown under salinity stress. In Vegetation Dynamics, Changing Ecosystems and Human Responsibility. Intechopen. 10.5772/intechopen.108710

(IV) Ekka, P., Patra, S., Upreti, M., Kumar, G., Kumar, A., Saikia, P., 2023. Land degradation and its impacts on biodiversity and ecosystem services. Land and environmental management through forestry, 77-101. 10.1002/9781119910527.ch4

(V) Filho, A.F.M., Azevedo, C.A.V., Azevedo, M.R.Q.A., Fernandes, J.D., Sousa, S., Fernandes, P.D., 2018. Economic viability of lettuce ('Lactuca sativa', L.) grown in hydroponic system with different optimized nutrient solutions. Australian Journal of Crop Science, 12(3), 422-429. 10.3316/informit.605303462329348

(VI) Folorunso, E.A., Schmautz, Z., Gebauer, R., Mraz, J., 2023. The economic viability of commercial-scale hydroponics: Nigeria as a case study. Heliyon, 9(8). 10.1016/j.heliyon.2023.e18979

(VII) Grover, M., Bodhankar, S., Sharma, A., Sharma, P., Singh, J., Nain, L., 2021. PGPR Mediated Alterations in Root Traits: Way Toward Sustainable Crop Production. Frontiers in Sustainable Food Systems, 4, 618230. 10.3389/fsufs.2020.618230

(VIII) Gupta, G., Parihar, S.S., Ahirwar, N.K., Snehi, S.K., Singh, V., 2015. Plant growth promoting rhizobacteria (PGPR): current and future prospects for development of sustainable agriculture. J Microb Biochem Technol, 7(2), 096-102. 10.4172/1948-5948.1000188

(IX) Hooks, T., Masabni, J., Sun, L., Niu, G., 2022. Effects of organic fertilizer with or without a microbial inoculant on the growth and quality of lettuce in an NFT hydroponic system. Technology in Horticulture, 2(1), 1-8. 10.48130/TIH-2022-0001

(X) Jagadish, R., Chowdappa, S., 2021. Diversity and antimicrobial potential of endophytic fungi from aromatic plants of Bhadra Wildlife Sanctuary, Western Ghats, Karnataka. Journal of Applied Biology & Biotechnology Vol. 9(05), pp. 1-12. 10.7324/JABB.2021.9501

(XI) Kader, A.A. 2002. Postharvest Technology of Horticultural Crops. California: University of California Agriculture and natural Resources Communication Services, Publication 3592. ISBN-13: 2001087610

(XII) Kalamulla, R., Karunarathna, S.C., Tibpromma, S., Galappaththi, M.C., Suwannarach, N., Stephenson, S.L., Yapa, N., 2022. Arbuscular mycorrhizal fungi in sustainable agriculture. Sustainability, 14(19). 10.3390/su141912250

(XIII) Moreira, V.D.A., Oliveira, C.E.D.S., Jalal, A., Gato, I.M.B., Oliveira, T.J.S.S., Boleta, G.H.M., Filho, M.C.M.T. 2022. Inoculation with Trichoderma harzianum and Azospirillum brasilense increases nutrition and yield of hydroponic lettuce. Archives of Microbiology, 204(7), 440. 10.1007/s00203-022-03047-w

(XIV) Mourouzidou, S., Ntinas, G.K., Tsaballa, A., Monokrousos, N., 2022. Introducing the power of plant growth promoting microorganisms in soilless systems: A promising alternative for sustainable agriculture. Sustainability, 15(7), 5959. 10.1007/s13199-019-00602-w

(XV) Mustafa, S., Kabir, S., Shabbir, U., Batool, R., 2019. Plant growth promoting rhizobacteria in sustainable agriculture: from theoretical to pragmatic approach., Symbiosis,78(2), 115-123. 10.1007/s13199-019-00602-w

(XVI) Oliveira, C.E.S., Jalal, A., Vitória, L.S., Giolo, V.M., Oliveira, T.J.S.S., Aguilar, J.V., Camargos, L.S., Brambilla, M.R., Fernandes, G.C., Vargas, P.F., Zoz, T., Filho, M.C.M.T. 2023. with Azospirillum brasilense Strains AbV5 and AbV6 Increases Nutrition, Chlorophyll, and Leaf Yield of Hydroponic Lettuce. Plants, 12(17), 3107. 10.3390/plants12173107

(XVII) Prasad, M., Srinivasan, R., Chaudhary, M., Choudhary, M., Jat, L.K., 2019. Plant growth promoting rhizobacteria (PGPR) for sustainable agriculture: perspectives and challenges. PGPR amelioration in sustainable agriculture, 129-157. 10.1016/B978-0-12-815879-1.00007-0

(XVIII) Ribeiro, V.P., Gomes, E.A., Sousa, S.M., Lana, U.G.P., Coelho, A.M., Marriel, I.E., Oliveira-Paiva, C.A., 2022. Co-inoculation with tropical strains of Azospirillum and Bacillus is more efficient than single inoculation for improving plant growth and nutrient uptake in maize. Archives of microbiology, 204(2), 143. 10.1007/s00203-022-02759-3

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Published

2026-03-17

How to Cite

Garrigues, M., & Barlisan Garrigues, R. (2026). SYNERGISTIC EFFECTS OF MICROBIAL INOCULANTS AND REDUCED NUTRIENTS ON HYDROPONIC LETTUCE PERFORMANCE AND PROFITABILITY . REVISTA DE AGRICULTURA NEOTROPICAL, 13(1). https://doi.org/10.32404/rean.v13i1.9766