Azospirillum brasilense INTERACTION EFFECTS WITH CAPTAN AND THIODICARB ON THE INITIAL GROWTH OF CORN PLANTS
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https://doi.org/10.32404/rean.v6i3.3133Abstract
The application of biological products containing diazotrophic bacteria in the seed has been highlighted as an alternative to reduce the use of nitrogen fertilizers; however, the chemical treatment of the seeds may negatively affect the survival rate of these microorganisms in the soil. The effects of the application of captan (fungicide) and thiodicarb (insecticide) in association with the inoculation of Azospirillum brasilense were investigated in this study for the corn crop. The research was conducted under greenhouse conditions at Universidade Estadual do Centro-Oeste (UNICENTRO), in Guarapuava, Paraná, Brazil, and under laboratory conditions at the Universidade Federal da Fronteira Sul (UFFS), in Laranjeiras do Sul, Paraná, Brazil. A completely randomized experimental design with eight treatments and four replicates was used. The treatments were as follows: control; A. brasilense; captan; thiodicarb; captan + thiodicarb; A. brasilense + captan; A. brasilense + thiodicarb; A. brasilense + captan + thiodicarb. The germination and seedling growth rates were evaluated in the laboratory. In the greenhouse were measured the growth of the roots and shoots until the V8 corn growth stages. The use of A. brasilense alone resulted in higher plant growth when compared to the control treatment, whereas the association of A. brasilense with captan and thiodicarb resulted in a similar response to the uninoculated and control treatments. These results suggest that there was a possible toxic effect of seed treatment with these products. The use of captan and thiodicarb interfere in the interaction of diazotrophic bacteria with root growth, affecting the germination rate and initial corn growth.
References
(I) Battistus, A.G., Hachmann, T.L., Mioranza, T.M., Muller, M.A., Madalosso, T., Favorito, P.A., Guimarães, V.F., Klein, J., Kestring, D., Inagaki, A.M., Bulegon, L.G., 2014. Synergistic action of Azospirillum brasilense combined with thiamethoxam on the physiological quality of maize seedlings. African Journal of Biotechnology, 13, 4501-4507.
(II) Brasil, 2009. Ministério da Agricultura e Reforma Agrária. Regras para análise de sementes. Brasília, 399 p.
(III) Campos, R.J., Araujo, R.S., Hungria, M., 2009. Nitrogen fixation with the soybean crop in Brazil: compatibility between seed treatment with fungicides and bradyrhizobial inoculants. Symbiosis, 48, 154-163.
(IV) Cassán, F., Perrig, D., Sgroy, V., Masciarelli, O., Penna, C., Luna, V., 2009. Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination, promote seed germination and early seedling growth in corn (Zea mays L.) and soybean (Glycine max L.). European Journal of Soil and Biology, 45, 28-35.
(V) Castro, G.S.A., Bogiani, J.C., Silva, M.G., Gazola, E., Rosolem, C.A., 2008. Tratamento de sementes de soja com inseticidas e um bioestimulante. Pesquisa Agropecuária Brasileira, 43, 1311-1318.
(VI) Dartora, J., Guimarães, V.F., Marini, D., Júnior, A.S.P., Cruz, L.M., Mensch, R., 2013. Influência do tratamento de sementes no desenvolvimento inicial de plântulas de milho e trigo inoculados com Azospirillum brasilense. Scientia Agraria Paranaensis, 12, 175-181.
(VII) Ferreira, M.M.M., Ferreira, G.B., Fontes, P.C.R., Dantas, J.P., 2015. Índice SPAD e teor de clorofila no limbo foliar do tomateiro em função de doses de nitrogênio e da adubação orgânica, em duas épocas de cultivo. Ceres, 53, 83-92.
(VIII) Fey, R., Vogel, G.F., Martinkoski, L., Richart, A., Rampim, L., Klein, J., 2015. Fixação biológica de nitrogênio e efeitos do tratamento químico das sementes na sua eficiência, in: Kuhn, O.J., (Org.), Ciências Agrárias: Tecnologias e Perspectivas, primeira ed. Marechal Cândido Rondon, Universidade Estadual do Oeste do Paraná, p. 139-170.
(IX) Fibach-Paldi, S., Burdman, S., Okon, Y., 2012. Key physiological properties contributing to rhizosphere adaptation and plant growth promotion abilities of Azospirillum brasilense. FEMS Microbiology Letters, 326, 99-108.
(X) Franche, C., Lindstrom, K., Elmerich, C., 2009. Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant and soil, 321, 35-59.
(XI) Gallori, E., Casalone, E., Colella, C.M., Daly, S., Polsinelli, M., 1991. 1,8-Naphthalic anhydride antidote enhances the toxic effects of captan and thiram fungicides on Azospirillum brasilense cells. Research in Microbiology, 142, 1005-1012.
(XII) Hungria, M., 2011. Inoculação com Azospirillum brasilense: inovação em rendimento a baixo custo. Londrina: Embrapa Soja, 37 p. (Documentos, 325).
(XIII) Hungria, M., Campo, R.J., Souza, E.M., Pedrosa, F.O., 2010. Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant and Soil, 331, 413-425.
(XIV) Kintschev, M.R., Goulart, A.C.P., Mercante, F.M., 2014. Compatibilidade entre a inoculação de rizóbios e fungicidas aplicados em sementes de feijoeiro-comum. Summa Phytopathologica, 40, 338-346.
(XV) Milani, K., Santos, O., Silva, M., Leziér, D., Barreira, B., Oliveira, A., 2013. Influência do meio de cultivo sobre a população e produção de exopolissacarídeos por Azospirillum brasilense Ab-V5. Biochemistry and Biotechnology Reports, 2, 212-215.
(XVI) Mohiuddin, M., Mohammed, M.K., 2013. Influence of fungicide (Carbendazim) and herbicides (2,4-D and Metribuzin) on non-target beneficial soil microorganisms of rhizospheric soil of tomato crop. IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT), 3, 47-50.
(XVII) Pereira, C.E., Oliveira, J.A., Oliveira, G.E., Rosa, M.C.M., Costa Neto, J., 2009. Tratamento fungicida via peliculização e inoculação de Bradyrhizobium em sementes de soja. Revista Ciências Agronômicas, 40, 433-440.
(XVIII) Reis Junior, F.B., Machado, C.T.T., Sodek, L., 2008. Inoculação de Azospirillum amazonense em dois genótipos de milho sob diferentes regimes de nitrogênio. Revista Brasileira de Ciência do Solo, 32, 1139-1146.
(XIX) Vogel, G.F., Fey, R., 2016. Estímulo do potencial germinativo e fisiológico de centeio e triticale por Azospirillum brasilense, submetidos ao tratamento químico de sementes. Scientia Agraria Paranaensis, 15, 493-498.
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