EFFECT OF SALINITY ON PHOTOSYNTHETIC AND ENZYMATIC ACTIVITIES AND TUBERIZATION YIELD IN THE GENOTYPE OF POTATO CULTIVAR AGRIA UNDER IN VITRO CONDITIONS

Visualizações: 772

Authors

  • Seyyed Nosrat Mousavi Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
  • Mostafa Ebadi Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. https://orcid.org/0000-0003-1123-7102
  • Mahdi Khorshidi Department of Biology, University of Damghan, Iran.
  • Hossein Hokmabadi Department of Biology, University of Damghan, Iran.‎
  • Nahid Masoudian Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.

DOI:

https://doi.org/10.32404/rean.v7i3.4028

Abstract

The aim of this study aimed to investigate the effect of salinity levels on physiological and biochemical properties and tuberization yield of potato, cultivar Agria, in MS culture medium. After preparation and cultivation of tuber, the lateral aerial buds were removed after a few months and cultured in MS medium. After that, healthy samples were prepared and induced at the above-mentioned salinity stress level and transferred to the culture medium. Two weeks later, an air sample was taken, and the physiological and biochemical properties studied in this study (photosynthetic pigments, proline, soluble sugars, anthocyanins, phenolic compounds, and the activity of some antioxidant enzymes) were measured. Finally, salinity stress assessment was evaluated and studied in a fluid environment. The effects of stress on different stages of seedling growth in vitro conditions and the activity of photosynthetic and enzymatic pigments were investigated using spectrophotometric measurements and in accordance with their protocols. Data analysis of variance showed that salinity reduces photosynthetic pigments and salinity to a concentration of 50 mM, it is resistant to the addition of compounds such as soluble sugars, proline and antioxidant activity but at higher concentrations (75 and 100 mM), the plant is likely to be severely damaged due to excessive growth in active oxygen species. Therefore, due to the increase in antioxidant activity, it indicates an increase in the removal of active oxygen species, this feature is often used as an indicator to increase salinity tolerance. Research on the different ecological conditions in Iran, research is needed on other potato cultivars and molecular studies.

References

(I) Abe, K., Yuki, S., Kogure, K., 1988. Strong attenuation of ischemic and postischemic brain edema in rats by a novel free radical scavenger. Stroke, 19(4), 480-485.

(II) Arab Ahmadi, H., Abaspour H., Gorbanli, M., 2014. The effect of salinity and ascorbate on seed germination percentage, growth parameters, proline and glycine betaien content in two cultivars of safflower (Carthamus Tinctorius L.). Journal of Plant Environmental Physiology, 9(1), 67- 76.

‎(III) Ben Hamed, K., Castagna, A., Salem, E., Ranieri, A., Abdelly, C., 2007. Sea fennel (Crithmum maritimum L.) under salinity conditions: a comparison of leaf and root antioxidant responses. Plant Growth Regulation, 53(3), 185-194.

(IV) Chen, G.X., Asada, K., 1989. Ascorbate peroxidase in tea leaves: occurence of two isozymes and the differences in their enzymatic and molecular properties. Plant & Cell Physiology, 30(7), 987-998.

(V) Christie, P.J., Alfenito, M.R., Walbot, V., 1994. Impact of low-temperature stress on general ‎phenylpropanoid and anthocyanin pathways: Enhancement of transcript abundance and anthocyanin ‎pigmentation in maize seedlings. Planta, 194(4), 541-549.‎

(VI) Daneshmand, F., Arvin, M.J., Kalantari, K.M, 2011. Reponses of wild species of potato to salt stress under in vitro culture. Iranian Journal of Biology, 24(1), 65-78.

(VII) Darreki, S., 2015. The effect of salinity stress on physiological ‎properties and activity of chickpea Azad cultivar antioxidant enzymes. Iranian Journal of Agricultural Research, 14(3), 470-483.

‎(VIII) Dubey, R.S., 1994. Protein synthesis by plants under stressful conditions, in: Pessaraki, M., (Ed.). Hand book ‎of plant and crop stress. Marcel Dekker, New York, p. 277-299.‎

(IX) Ebadi, M., Majd, A., Fallahian, F., 2011. Evaluation of developmental-cellular process of potato plant in tissue culture, cell and semi-continuous and continuous bioreactors. Thesis Islamic Azad University, Science and Research Branch, Iran.

(X) Fakhimi, R.H., 2018. In vitro study of the effect of salicylic acid on some growth and biochemical properties of potato cultivar Agria under salinity stress. Journal of Plant Environmental Physiology, 13(5), 1-12.

(XI) Gao, Z.F., Sagi, M., Lips, S.H., 1998. Carbohydrate metabolism in leaves and assimilate partitioning in ‎fruits of tomato (Lycopersicon esculentum L.) as affected by salinity. Plant Science, 1(3), 149-159.

(XII) Hipskind, J., Wood, K., Nicholson, R.L., 1996. Localized stimulation of anthocyanin accumulation and ‎delineation of pathogen ingress in maize genetically resistant to Bipolaris maydis Race O. Physiological and Molecular Plant Pathology, 49(4), 247-256.‎

(XIII) Hung, I., Redman, R.E., 1995. Solute adjustment to salinity and calcium supply in cultivated and wild ‎barley. Journal of Plant Nutrition, 18(7), 1371-1389.‎

(XIV) Karimi, G.H., Assareh, M.H., 2012. Effects of salinity stress on some physiological characteristics of kochia prostate. Iranian Journal of Range and Desert Research, 18(4), 537-546.

(XV) Khatamsaz, M., 1998. Solanaceae (No. 24), in: Assadi, M. et al. (Ed.). Flora of Iran. Research Institute of Forests and Rangelands, 114 p.

(XVI) Khavarinejad, R.A., Chaparzadeh, N., 1998. The effects of NaCl and CaCl2 on photosynthesis and growth of ‎alfalfa plants. Photosynthetica, 35, 461-466.‎

(XVII) Khorshidi Benam, M.B., Rahimzadeh Khoii, F., Mirhadi, M.J., Nour-Mohamadi, G., 2002. Study of drought stress effects in different growth stages on potato cultivars. Iranian Journal of Crop Sciences, 4(1), 48-59.

(XVIII) Koca, H., Bor, M., Ozdemir, F., Turkan, I., 2007. The effect of salt stress on lipid peroxidation, antioxidative ‎enzymes and proline content of sesame cultivars. Environmental and Experimental Botany, 60(3), 344-351.‎

(XIX) Moeini, A., Kahrizi, H., 2003. Plant cultivation translation. Student Mobilization Organization, SAMT publisher, 66-69.

(XX) Mollassiotis, A.N., Sotiropoulos, T., Tanou, G., Kofidis, G., Diamantidis, G., Therios, I., 2006. Antioxidant and ‎anatomical responses in shoot culture of the apple rootstock MM 106 Treated with NaCl, KCl, mannitiol or ‎sorbitol. Biologia Plantarum, 50(3), 331-338.‎

(XXI) Movahhedi Dehnavi, M., Niknam, N., Behzadi, Y., Mohtashami, R., Bagheri, R., 2017. Comparison of physiological responses of linseed (Linum usitatissimum L.) to drought and salt stress and salicylic acid foliar application. Iranian Journal of Plant Biology, 9(3), 39-62.

(XXII) Munns, R., 2005. Genes and salt tolerance: Bringing them together. New Phytol, 167, 645-663.

(XXIII) Nakano, Y., Asada, K., 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology, 22(5), 867-880.

(XXIV) Saeidipour, S., 2015. Salinity effects on osmotic potential, soluble proteins and carbohydrates concentration in rice (Oryza sativa) genotypes at seedling stage. Applied Field Crops Research (Pajouhesh & Sazandegi), 109, 1-8.

(XXV) Sairam, R.K., Deshmukh, P.S., Saxena, D.C., 1998. Role of antioxidant systems in wheat genotype tolerance to ‎water stresses. Biologia Planta, 41(3), 387-394.‎

(XXVI) Shahbazi, P., 2015. The effect of salinity stress on antioxidant activity and seedling growth in Safflower (Carthamus ‎tinctorius L.) genotypes, 7(1), 10-21.

(XXVII) Szabados L., Kovacs H., Zilberstein A., Bouchereau A., 2011. Plants in extreme environments: importance of protective compounds in stress tolerance. Advances in Botanical Research, 57, 105-150.

‎(XXVIII) Zarghami, R., 2009. The effect of culture medium and used hormones on root formation and growth of single nodes from potato meristem culture. First Biotechnology Conference, Tehran, 34-55.

Downloads

Published

2020-09-09

How to Cite

Mousavi, S. N., Ebadi, M., Khorshidi, M., Hokmabadi, H., & Masoudian, N. (2020). EFFECT OF SALINITY ON PHOTOSYNTHETIC AND ENZYMATIC ACTIVITIES AND TUBERIZATION YIELD IN THE GENOTYPE OF POTATO CULTIVAR AGRIA UNDER IN VITRO CONDITIONS. REVISTA DE AGRICULTURA NEOTROPICAL, 7(3), 8–19. https://doi.org/10.32404/rean.v7i3.4028