CULTURE MEDIA EVALUATION ON THE Leucoagaricus gongylophorus AND Escovopsis sp. FUNGI DEVELOPMENT
Visualizações: 522DOI:
https://doi.org/10.32404/rean.v8i3.5869Abstract
This study aimed to evaluate the in vitro interference of different culture media, Czapek Dox Agar, Nutrient Agar, Starch Casein agar, Malt Extract Broth Base, Nutrient Broth, and the medium containing Peptone, Malt, Agar and crushed peach palm pulp (Bactris gasipaes) and tucumã (Astrocaryum aculeatum) on the fungus Leucoagaricus gongylophorus and the parasitic fungus Escovopsis sp. growth. The fungi were inoculated in different culture media and then kept in acclimatized chambers at 28 ºC in the dark for 42 days, being analyzed at weekly intervals, totaling six evaluations. Fungal colonies were evaluated considering the factor "f" (cm), obtained from the product between the width (W) and length (L) of the diameter of the colonies (f = W x L). The results showed that the symbiotic fungus and the leafcutter ant parasitic fungus showed a growth increase compared to the media provided in the plates, and the media added with tucumã peel, tucumã peel+pulp, fresh peach palm, and cooked peach palm had a significant effect in the L. gongylophorus fungus development. However, these treatments were not promising for the Escovopsis sp. fungus compared to other culture media.
References
(I) Azevedo, J.L.; Costa, S.O.P., 1973. Exercícios práticos de genética, São Paulo: EDUSP, 288 p.
(II) Borba, R.S.; Loecki, A.E.; Bandeira, J.M.; Moraes, C.L.; Centenaro. E.D., 2006. Crescimento do fungo simbionte de formigas cortadeiras do gênero Acromyrmex em meios de cultura com diferentes extratos. Ciência Rural, Santa Maria, 36(3),725-730. DOI: https://doi.org/10.1590/S0103-84782006000300002.
(III) Barbosa, F.H.F; Barbosa, L.P.J.L., 2010. Alternativas Metodológicas em Microbiologia: viabilizando atividades práticas. Revista de Biologia e Ciências da Terra, 10(2), 134-143. http://joaootavio.com.br/bioterra/workspace/uploads/artigos/artigo_15_v10_n2-51562daa0b616.pdf. (acessado 10 janeiro de 2020).
(IV) Camargo, R.S.; Forti, L.C.; Rocha, M.M.; Matos, C.A.O.; Lopes, J.F.; Andrade, A.P.P.; Verza, S.S., 2003. The effect of plant diversity on fungus garden development and foraging behavior of leaf-cutting ants (Hymenoptera: Formicidae)., 2003. Sociobiology, Chico, 42(2), 1-10. http://www.csuchico.edu/biol/Sociobiology/volume/sociobiologyv42n22003.html#11. (acessado 10 de janeiro de 2020).
(V) Cantarelli, E.B.; Costa, E.C.; Pezzutti, R.V.; Zanetti, R.; Fleck, M.D. 2019. Damage by Acromyrmex spp. to an initial Pinus taeda L. planting. Floresta e Ambiente, 26(4), 1-7. https://doi.org/10.1590/2179-8087.006016.
(VI) Currie, C.R.; Mueller, U.G.; Malloch, D., 1999a. The agricultural pathology of ant fungus gardens. Proceedings of the National Academy of Sciences, 96(14), 7998-8002. https://doi.org/10.1038/19519
(VII) Currie, C.R.; Scott, J.A.; Summerbell, R.C.; Malloch, D. 1999b. Fungus-growing ants use antibiotic-producing bacteria to control garden parasites. Nature, 398, 701-704. https://doi.org/10.1038 / 19519
(VIII) Castelani, A., 1939. Viability of mold culture of fungi in destilled water. J Trop Med & Hyg.
(IX) Demirtas, A.; Ozturk, H.; Piskin, I. 2018. Overview of plant extracts and plant secondary metabolites as alternatives to antibiotics for modification of ruminal fermentation. Ankara universitesi Veteriner Fakultesi Dergisi, 65(2)13-217. https://doi.org/10.1501/Vetfak_0000002849
(X) Della Lucia, T.M.C., Souza, D.J. Importância e história de vida das formigas cortadeiras. In: Della Lucia, T.M.C. (Ed.). Formigas cortadeiras – da Bioecologia ao Manejo, Viçosa, MG: Editora UFV, 2011. p. 13-26.
(XI) Della Lucia, T.M.C.; Gandra, L.C.; Guedes, R.N. 2014. Managing leaf‐cutting ants: peculiarities, trends and challenges. Pest management science, 70(1), 14-23. https://doi.org/10.1002/ps.3660.
(XII) Ferreira, D. F. 2014 Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e Agrotecnologia, Lavras, 38(2), 109-112. https://doi.org/10.1590/S1413-70542014000200001
(XIII) Garcia. L.F.M, Sagrilo. M.R.; Souza-Filho. O.C.; Ribeiro. E.E. 2013. The In Vitro Genotoxic Effect of Tucuma (Astrocaryum aculeatum), an Amazonian Fruit Rich in Carotenoids. Journal of Medicinal Food, 16 (2), 1013-1021. https://doi.org/10.1089/jmf.2012.0287
(XIV) Koch, A.L. 1975. The kinects of mycelial growth. Journal of General Microbiology, 89(2), 209-216. DOI: https://doi.org/10.1099/00221287-89-2-209
(XV) Loeck, A.E.; Pierobom, C.R.; Gusmão, L.G.; Afonso, A.P. 2004. Growth of symbiont fungi of some higher Attini ants in mineral médium. Ciência Rural, Santa Maria, 34(1), 79-82. DOI: https://doi.org/10.1590/S0103-84782004000100012
(XVI) Man, T.J.B.; Stajich, J.E.; Kubicekc, C.P.; Teiling, C.; Chenthamara, K.; Atanasova, L.; Druzhinina, I.S.; Levenkova, N.; Birnbaum, S.S.L.; Barribeau, S.M.; Bozick, B.A; Suen, G.; Currie, C.R.; Gerardo, N.M. 2016. Small genome of the fungus
Escovopsis weberi, a specialized disease agent of ant agriculture. Proceedings of the National Academy of Sciences of the United States of America, Washington, 113(13), 3567-3572. DOI: https://doi.org/10.1073/pnas.1518501113.
(XVII) Moreno, L.,F.; Feng, P.; Weiss, V.A.; Vicente, V.A.; Stielow, B.; Hoog, S. 2017. Phylogenomic analyses reveal the diversity of laccase-coding genes in Fonsecaea genomes. Plos one, 12(1), 171-291. DOI: https://doi.org/10.1371/journal.pone.0171291.
(XVIII) Mueller, U.G.; Kardish, M.R.; Ishak, H.D.; Wright, A.M.; Solomon, S.E.; Bruschi, S.M.; Carlson, A.L.; Bacci Jr, M. 2018. Phylogenetic patterns of ant–fungus associations indicate that farming strategies, not only a superior fungal cultivar, explain the ecological success of leafcutter ants. Molecular Ecology, 27(10), 2414–2434. DOI: https://doi.org/10.1111/mec.14588.
(XIX) Nepomucena, R.M.P. 2010. Avaliação do potencial microbiano do crescimento e de secreção de lacase do fungo amazônico lentinus crinitus (L. ex Fr.) (Dissertação de Mestrado) Biotecnologia e Recursos Naturais, Universidade Estadual do Amazonas, Manaus, AM. http://repositorioinstitucional.uea.edu.br//handle/riuea/2216. (acessado 10 de janeiro de 2020).
(XX) Pagnocca, F.C.; Rodrigues. A.; Jr. Bacci, M. 2011. Microrganismos associados as formigas-cortadeiras. In: Della lucia, T.M.C. (Ed.) Formigas-cortadeiras: da bioecologia ao manejo. Viçosa: Editora da UFV, 262-283.
(XXI) Reynolds, H.T.; Currie, C.R. 2004. Pathogenicity of Escovopsis weberi: The parasite of the attine ant-microbe symbiosis directly consumes the ant-cultivated fungus. Mycologia, Lawrence, 96(5), 955–959. PMID: 21148916.
(XXII) Seipke, R., Crossman, L., Drou, N., Darren, H., Mervyn, J., Caccamo, M.; Matthew, I. 2011. Draft genome sequence of Streptomyces Strain S4, a symbiont of the leafcutting ant Acromyrmex octospinosus. J Bacteriol, 193, 4270– 4271. https://doi.gor/10.1128 / jb.05275-11
(XXIII) Siqueira, C.G., Bacci, M., Pagnocca, F. C., Bueno, O. C., Hebling, M.J. 1998. Metabolism of plant polysaccharides by Leucoagaricus gongylophorus, the symbiotic fungus of the leafcutting ant Atta sexdens L. Applied and Environmental Microbiology, 64, 4820-4822. https://doi.org/10.1128/AEM.64.12.4820-4822.1998.
(XXIV) Scalbert, A. 1991. Antimicrobial properties of tannins. Phytochemistry, 30(12), 3875-3883. https://doi.org/10.1016/0031-9422(91)83426-L.
(XXV) Victor, S.R., Crisostomo, F.R., Bueno, F.C., Pagnocca, F.C., Fernandes, J.B., Correa, A.G.; Silva, M.F.G.F. 2001. Toxicity of synthetic piperonyl compounds to leaf-cutting ants and their symbiotic fungus. Pest Management Science, 57(7), 603-608. https://doi.org/10.1002/ps.333.
(XXVI) Zanetti, R.; Zanuncio, J.C.; Santos, J.C.; Silva, W.L.P.; Ribeiro, G.T.; Lemes, P.G. 2014. An overview of integrated management of leaf-cutting ants (Hymenoptera: Formicidae) in Brazilian forest plantations. Forests, 5(3), 439-454. https://doi.org/10.3390/f5030439
Downloads
Published
How to Cite
Issue
Section
License
The authors retain the rights to the manuscripts and, therefore, are free to share, copy, distribute, perform and publicly communicate the work under the following conditions:
Acknowledge work credits in the manner specified by the author or licensor (but not in a way that suggests that you have their support or that they support their use of their work).
REVISTA DE AGRICULTURA NEOTROPICAL (ISSN 2358-6303) is under license https://creativecommons.org/licenses/by/4.0/
The State University of Mato Grosso do Sul, Sustainable Development Center of Bolsão Sul-Mato-grossense (CEDESU), of the University Unit of Cassilândia (UUC), preserves the patrimonial rights (copyright) of the published works and favors and allows their reuse under the license as mentioned above.
------------
The journal reserves the right to make normative, orthographic, and grammatical alterations in the originals, to maintain the cult standard of the language, respecting, however, the style of the authors.
Final proofs will be sent to the authors.
Published works become the property of the journal. The opinions expressed by the authors of the manuscripts are their sole responsibility.