HYDRATION LEVELS ON CONIDIAL PRODUCTION OF Metarhizium rileyi (Ascomycota) IN SOLID GROWING MEDIUM
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https://doi.org/10.32404/rean.v6i3.2899Abstract
This study aimed to evaluate the conidial production of Metarhizium rileyi in rice with different water volumes. The bioassay was composed by completely randomized design (CRD), with four treatments (20, 30, 40 and 50 mL of distilled water), being added 100 g of rice thin and long, making a total of 10 plastic bags per treatment, which were autoclaved for 15 minutes at 1.0 atm pressure, to 120 ºC. After the cooling of the rice, were added in each plastic bag, 2.0 mL of suspension containing 1 × 108 conidia mL-1. Then the bags were incubated for ten days in a germination chamber (BOD type) at 25 °C (±1 °C), 80% (±10%) relative humidity and 12h photoperiod to promote conidial germination and growth of the fungus, being performed a mild agitation every two days. The use of higher water volume resulted in greater conidial production and greater number of viable conidia. However, the highest rate of conidia germination was obtained with the use of 30 mL of water, is this the volume of water that corresponds to the best results.
References
(I) Alves, S.B., Lopes, R.B., Vieira, S.A., Tamai, M.A., 2008. Fungos entomopatogênicos usados no controle de pragas na América Latina, in: Alves, S.B., Lopes, R.B., (Ed.), Controle microbiano de pragas na América Latina. FEALQ, Piracicaba, p. 69-110.
(II) Alves, S.B., Pereira, R.M., 1998. Produção de fungos entomopatogênicos, in: Alves, S.B., Lopes, R.B., (Ed.), Controle microbiano de insetos. FEALQ, Piracicaba, p. 845-870.
(III) Almeida, J.E.M., Batista Filho, A.B., 2006. Microorganismos no controle de pragas, in: Pinto, A.S., Nava, D.E., Rossi, M.M., Malerbo-Souza, D.T., (Ed.), Controle Biológico de pragas: na prática. CP2. Piracicaba, p. 35-44.
(IV) Bhanu Prakash, G.V., Padmaja, V., Siva Kiran, R.R., 2008. Statistical optimization of process variables for the large-scale production of Metarhizium anisopliae. Bioresource Technology, 99(6), 1530-1537.
(V) Bhering, L.L., 2017. Rbio: A Tool For Biometric And Statistical Analysis Using The R Platform. Crop Breeding and Applied Biotechnology, 17, 187-190.
(VI) Boucias, D.G., Stokes, C., Suazo, A., Funderbuck, J., 2000. AFLP analysis of the entomopathogen Nomuraea rileyi. Mycologia, 92(4), 638-648.
(VII) Céspedes, I., Del Pozo, E., Garcia, I., Méndez, A., 2008. Efecto de la temperatura sobre el hongo entomopatógeno Nomuraea rileyi (Farlow) Samson y su efectividad sobre Spodoptera frugiperda J. E. Smith. Revista Protección Vegetal, 23(3), 176-182.
(VIII) Chaudhari, C.S., Chandele, A.G., Pokharkar, D.S., Dethe, M.D., Firake, D.M., 2015. Pathogenicity of Different Isolates of Entomopathogenic Fungus, Nomuraea rileyi (Farlow) Samson Against Tobacco Caterpillar, Spodoptera litura (Fabricius). Proceedings of the National Academy of Sciences, 2, 1-7.
(IX) Chen, H., Yin, Y.P., Li, Y., Mahmud, M.S., Wang, Z.K., 2012. Identification and analysis of genes differentially expressed in the Spodoptera litura fat body in response to the biocontrol fungus, Nomuraea rileyi. Comparative Biochemistry and Physiology, 163, 203-210.
(X) Crowe, J.H., Crowe, L.M., Deamer, D.W., 1982. Hydration dependent phase changes in biological membrane, in: Biophysics of Water, F. Fanks & S. Mathias, (Ed.), Wiley, Chichester, p. 295-299.
(XI) Devi, P.S.V., Prasad, Y.G., Chowdary, D.A., Rao, L.M., Balakrishnan, K., 2003. Identification of virulent isolates of the entomopathogenic fungus Nomuraea rileyi (F) Samson for the management of Helicoverpa armigera and Spodoptera litura. Mycopathologia, 156(4), 365-373.
(XII) Devi, P.S., Chowdary, A., Prasad, Y.G., 2001. Cost-effective multiplication of the entomopathogenic fungus Nomuraea rileyi (F) Samson. Mycopathologia, 151(1), 35-39.
(XIII) Faria, M., Wraight, S.P., 2007. Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types. Biological Control, 43, 237-256.
(XIV) Freitas, A.F., Loureiro, E.S., Almeida, M.E.B., Pessoa, L.G.A., 2014. Rendimento de conídios e germinação de diferentes isolados de Metarhizium anisopliae (Metsch.) Sorok. (Ascomycota: Clavicipitaceae) cultivados em arroz. Arquivos do Instituto Biológico, 81(1), 75-78.
(XV) Ignoffo, C.M., 1981. The fungus Nomuraea rileyi as a microbial insecticide, in: Burges, H.D., (Ed.), Microbial control of pests and plants diseases 1970-1980. Academic Press, p. 413-538.
(XVI) Jenkins, N.E., Heviofo, G., Langewald, J., 1998. Development of mass production technology for aerial conidia for use as mycopesticides. Biocontrol News and Information, 31(2), 21-31.
(XVII) Kepler, R.M., Humber, R.A., Bischoff, J.F., Rehner, S.A., 2014. Clarification of generic and species boundaries for Metarhizium and related fungi through multigene phylogenetics. Mycologia, 106, 464-480.
(XVIII) Lacey, L.A., Frutos, R., Kaya, H.K., Vail, P., 2001. Insect pathogens as biological control agents: Do they have a future? Biological Control, 2(3), 230-248.
(XIX) Loureiro, E.S., Batista Filho, A., Almeida, J.E.M., Pessoa, L.G.A., 2005. Produção de isolados de Metarhizium anisopliae selecionados para o controle de Mahanarva fimbriolata (Stal, 1854). Arquivos do Instituto Biológico, 72(4), 469-472.
(XX) Méndez, A., Del Pozo, E., Garcia, I., 2009. Producción masiva de Nomuraea rileyi (Farlow) Samson mediante una alternativa de cultivo bifásico. Revista Protección Vegetal, 24(3), 156-161.
(XXI) Méndez, A., Del Pozo, E., Garcia, I., González, A., 2010. Evaluación de sustratos sólidos para la producción masiva de Nomuraea rileyi (Farlow) Samson. Revista Protección Vegetal, 25(2), 108-112.
(XXII) Perinotto, W.M.S., Terra, A.L.M., Angelo, I.C., Fernandes, E.K.K., Golo, P.S., Camargo, M.G., Bittencourt, V.R.E.P., 2012. Nomuraea rileyi as biological contro agents of Rhipicephalus microplus tick. Parasitology Research, 111(4), 1743-1748.
(XXIII) Sahayaraj, K., Karthick, S., 2008. Mass production of entomopathogenic fungi using agricultural products and byproducts. African Journal Biotechnology, 7(12), 1907-1910.
(XXIV) Song, Z.Y., Jiang, W., Yin, Y.P., Wang, Z.K., 2016. Polarity proteins Mrcdc24 and Mrbem1 required for hypha growth and microsclerotia formation in Metarhizium rileyi. Biocontrol Science Technology, 26, 733-745.
(XXV) Sujii, E.R., Carvalho, V.A., Tigano, M.S., 2002. Cinética da esporulação e viabilidade de conídios de Nomuraea rileyi (Farlow) Samson sobre cadáveres da lagarta-da-soja, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae), em condições de campo. Neotropical Entomology, 31, 85-90.
(XXVI) Suwannakut, S., Boucias, D.G., Wiwat, E.C., 2005. Genotypic analysis of Nomuraea rileyi collected from various noctuid hosts. Journal of Invertebrate Pathology, 90(3), 169-176.
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