ENCAPSULATED BAITS CONTAINING ZINC SULFATE AND Trichoderma harzianum REDUCE FUNGAL GARDEN IN Atta sexdens COLONIES

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Authors

  • Daniella Gonçalves da Silva Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.
  • Aldemir Lucena Júnior Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.
  • Renato de Almeida Sarmento Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.
  • Cynthia Lhourrana Santos Silva Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.
  • Amanda Caroline Teles Tenório Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.
  • Danival José de Souza Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.
  • Márcio Silva Melo Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.

DOI:

https://doi.org/10.32404/rean.v7i3.5064

Abstract

The aim of the study was to test the effects of various concentrations of zinc sulfate (ZnSO4) on the radial growth of Trichoderma harzianum and Leucoagaricus gongylophorus in Petri dishes. In addition, we investigated the acceptance of baits containing live propagules of the encapsulated T. harzianum fungus and baits containing ZnSO4 by foraging leafcutter worker ants, A. sexdens, as well as their effects on the colonies of these ants. For the in vitro test, the design was completely randomized using ten replicates of a 2 × 7 factorial scheme: two species of fungi (T. harzianum and L. gongylophorus) and seven concentrations of ZnSO4. For bait acceptance tests, four treatments were used: baits without the mycelium and ZnSO4 (T1, control), baits containing encapsulated T. harzianum mycelium (T2), baits containing ZnSO4 (T3), and baits containing zinc sulfate and the encapsulated T. harzianum (T4). Each treatment was tested in duplicates using eight colonies. In the in vitro test, a ZnSO4 concentration of 0.25 g/L was sufficient to completely inhibit the development of L. gongylophorus without affecting the development of T. harzianum. In the bait acceptance test, the workers accepted ~55% of the bait combination of ZnSO4 and T. harzianum. This suggests the potential of treatment T4 in controlling leafcutter ants. Moreover, treatment T4 is environmentally friendly. 

Author Biographies

Daniella Gonçalves da Silva, Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.

Programa de Pós-graduação em Ciências Florestais e Ambientais.

Aldemir Lucena Júnior, Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.

Programa de Pós-graduação em Ciências Florestais e Ambientais.

Renato de Almeida Sarmento, Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.

Produção Vegetal/Fitossanidade/Entomologia

Cynthia Lhourrana Santos Silva, Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.

Programa de Pós-graduação em Produção Vegetal

Amanda Caroline Teles Tenório, Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.

Engenharia de Bioprocessos e Biotecnologia

Danival José de Souza, Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.

Produção Vegetal/Fitossanidade (Entomologia)

Márcio Silva Melo, Universidade Federal do Tocantins, Campus de Gurupi, Gurupi, Tocantins, Brasil.

Programa de Pós-graduação em Produção Vegetal

References

(I) Anahid, S., Yaghmaei, S., Ghobadinejad, Z., 2011. Heavy metal tolerance of fungi. Scientia Iranica, 18(3), 502-508.

(II) Aylward, F.O., Burnum-Johnson, K.E., Scott, J.J., Suen, G., Tringe, S.G., Adams, S.M., Barry, K.W., Nicora, C.D., Piehowski, P.D., Purvine, S.O., Starrett, G.J., Goodwin, L.A., Smith, R.D., Lipton, M.S., Currie, C.R., 2012. Metagenomic and metaproteomic insights into bacterial communities in leafcutter ant fungus gardens. Multidisciplinary Journal of Microbial Ecology, 6(9), 1688-1701.

(III) Cumagun, C.J.R., 2014. Advances in Formulation of Trichoderma for Biocontrol, in: Gupta, V., Schmoll, M., Herrera-Estrella, A., Upadhyay, R.S., Druzhinina I., Tuohy, M., (Eds.). Biotechnology and Biology of Trichoderma. Elsevier, Amsterdam, 527-531.

(IV) Datta, B., 2015. Heavy metal tolerance of filamentous fungi isolated from metal- contaminated soil. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 17(4), 965-968.

(V) Della Lucia, T.M.C., 2011. Formigas-Cortadeiras: da Bioecologia ao Manejo, primeira ed. UFV, Viçosa.

(VI) Della Lucia, T.M.C., Gandra, L., Guedes, R., 2014. Managing leaf-cutting ants: peculiarities, trends and challenges. Pest Management Science, 70(1), 14-23.

(VII) Fine Licht, H.H., Boomsma, J.J., Tunlid, A., 2014. Symbiotic adaptations in the fungal cultivar of leaf-cutting ants. Nature Communications, 5, 5675.

(VIII) Folgarait, P., Gorosito, N., Poulsen, M., Currie, C.R., 2011. Preliminary in vitro insights into the use of natural fungal pathogens of leaf-cutting ants as biocontrol agents. Current Microbiology, 63(3), 250-258.

(IX) FSC, 2017. FSC® List of ‘highly hazardous’ pesticides. https://ic.fsc.org/file-download.fsc-list-of-highly-hazardous-pesticides.a-2951.pdf (Accessed July 12, 2020).

(X) Gadd, G.M., Raven, J.A., 2010. Geomicrobiology of Eukaryotic Microorganisms. Geomicrobiology Journal, 27(6-7), 491-519.

(XI) He, L., Liu, Y., Mustapha, A., Lin, M., 2011. Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum. Microbiological Research, 166(3), 207-215.

(XII) Lange, L., Grell, M.N., 2014. The prominent role of fungi and fungal enzymes in the ant-fungus biomass conversion symbiosis. Applied Microbiology and Biotechnology, 98(11), 4839-4851.

(XIII) Lewis, J.A., Papavizas, G.C., 1985. Characteristics of alginate pellets formulated with Trichoderma and Gliocladium and their effect on the proliferation of the fungi in soil. Plant Pathology, 34(4), 571-577.

(XIV) Menezes, J.P., Junges, E., Blume, E., Pereira, M.E., 2010. Toxicologia do biopreparado à base de Trichoderma sp. (Isolado UFSM T17) administrado em mamífero. Revista da Faculdade de Zootecnia, Veterinária e Agronomia (FZVA), 17(1), 28-40.

(XV) Montoya-Lerma, J., Giraldo-Echeverri, C., Armbrecht, I., Farji-Brener, A., Calle, Z., 2012. Leaf-cutting ants revisited: Towards rational management and control. International Journal of Pest Management, 58(3), 225-247.

(XVI) Mukherjee, M., Mukherjee, P.K., Horwitz, B.A., Zachow, C., Berg, G., Zeilinger, S., 2012. Trichoderma-plant-pathogen interactions: advances in genetics of biological control. Indian Journal of Microbiology, 52(4), 522-529.

(XVII) Mukherjee, P.K., Horwitz, B.A., Herrera-Estrella, A., Schmoll, M., Kenerley, C.M., 2013. Trichoderma research in the Genome Era. Annual Review of Phytopathology, 51(1), 105-129.

(XVIII) Nascimento, M.O., Almeida Sarmento, R., Santos, G.R., Oliveira, C.A., Souza, D.J., 2017. Antagonism of Trichoderma isolates against Leucoagaricus gongylophorus (Singer) Möller. Journal of Basic Microbiology, 57(8), 699-704.

(XIX) Savi, G.D., Bortoluzzi, A.J., Scussel, V.M., 2013. Antifungal properties of Zinc-compounds against toxigenic fungi and mycotoxin. International Journal of Food Science and Technology, 48(9), 1834-1840.

(XX) Souza, M.D., Peres Filho, O., Caldeira, S.F., Pelissari, A.L., Dorval, A., 2011. Análise de agrupamento e regressão não-linear aplicados ao crescimento in vitro de Leucoagaricus gongylophorus (Singer) Möller em meios de cultura acrescido com diferentes extratos vegetais. Biotemas, 24(4), 85-93.

(XXI) Ward, P.S., Brady, S.G., Fisher, B.L., Schultz, T.R., 2015. The evolution of myrmicine ants: phylogeny and biogeography of a hyperdiverse ant clade (Hymenoptera: Formicidae). Systematic Entomology, 40(1), 61-81.

(XXII) Zanuncio, J.C., Lemes, P.G., Antunes, L.R., Maia, J.L.S., Mendes, J.E.P., Tanganelli, K.M., Salvador, J.F., Serrão, J.E., 2016. The impact of the Forest Stewardship Council (FSC) pesticide policy on the management of leaf-cutting ants and termites in certified forests in Brazil. Annals of Forest Science, 73(2), 205-214.

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Published

2020-09-09

How to Cite

da Silva, D. G., Lucena Júnior, A., Sarmento, R. de A., Santos Silva, C. L., Teles Tenório, A. C., de Souza, D. J., & Melo, M. S. (2020). ENCAPSULATED BAITS CONTAINING ZINC SULFATE AND Trichoderma harzianum REDUCE FUNGAL GARDEN IN Atta sexdens COLONIES. REVISTA DE AGRICULTURA NEOTROPICAL, 7(3), 46–52. https://doi.org/10.32404/rean.v7i3.5064

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Vol. 7 No. 3 (2020): REVISTA DE AGRICULTURA NEOTROPICAL

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