TETRAZOLIUM AND INTERACTION OF TEMPERATURE AND LIGHT UNDER SEED GERMINATION IN Ormosia arborea (FABACEAE)
Visualizações: 719DOI:
https://doi.org/10.32404/rean.v8i1.5206Abstract
The study aimed to define the best conditions to conduct germination tests for Ormosia arborea seeds and assess the viability of seeds using the tetrazolium test. The germination tests were conducted at temperatures of 10, 15, 20, 25, 30, and 35 °C and alternating temperatures of 20-30 °C, in the presence or absence of light. For the tetrazolium test, seeds were immersed in tetrazolium solution 0.075%, at 35 °C for three hours, and then the viability was evaluated. It was verified that the seeds did not present absolute photosensitivity. The tetrazolium test was effective in separating the seeds into two categories concerning seed viability, viable and nonviable. It was concluded that O. arborea seeds germinate in the presence or absence of light in a wide range of temperatures. Temperatures of 25 and 30 ºC and alternating temperatures of 20-30 ºC are indicated to conduct germination tests. The tetrazolium test was effective in assessing seed viability.
References
(I) Abbade, L.C., Takaki, M. 2014. Teste de tetrazólio para avaliação da qualidade de sementes de Tabebuia roseoalba (Ridl.) Sandwith - Bignoniaceae, submetidas ao armazenamento. Revista Árvore, 38(2), 233-240. DOI: https://doi.org/10.1590/S0100-67622014000200003.
(II) Araújo, J.O., Pinheiro, D.T., Dias, D.C.F.S., Hilst, P.C., Medeiros, A.D. 2019. Adequacy of the tetrazolium test to evaluate the viability of Jatropha curcas L. seeds. Journal of Seed Science, 41(4), 470-477. DOI: https://doi.org/10.1590/2317-1545v41n4223299.
(III) Bewley, J.D., Black, M. 1982. Physiology and biochemistry of seeds in relation to germination: viability, dormancy, and environmental control, v. 2. New York, Springer.
(IV) Brancalion, P.H.S., Novembre, A.D.L.C., Rodrigues, R.R. 2010. Temperatura ótima de germinação de sementes de espécies arbóreas brasileiras. Revista Brasileira de Sementes, 32(4), 15-21. DOI: https://doi.org/10.1590/S0101-31222010000400002.
(V) Brasil. 2009. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Brasília, MAPA.
(VI) Cardoso, V.J.M. 2004. Dormência: estabelecimento do processo. In: Ferreira, A.G., Borguetti, F. (Org.) Germinação: do básico ao aplicado. Porto Alegre, Artmed, p. 95-134.
(VII) Costa, C.J., Santos, C.P. 2010. Teste de tetrazólio em sementes de leucena. Revista Brasileira de Sementes, 32(2), 66-72. DOI: https://doi.org/10.1590/S0101-31222010000200008.
(VIII) Daibes, L.F., Zupo, T., Silveira, F.A.O, Fidelis, A. 2017. A field perspective on effects of fire and temperature fluctuation on Cerrado legume seeds. Seed Science Research, 27(2), 74-83. DOI: http://dx.doi.org/10.1017/S096025851700006X.
(IX) Fenner, M. 2000. Seeds: the ecology and regeneration in plant communities, second ed. New York: CABI Publishing.
(X) Ferreira, S.A.N., Gentil, D.F.O. 2017. Seed germination at different stratification temperatures and development of Phytelephas macrocarpa Ruiz & Pavón seedlings. Journal of Seed Science, 39(1), 20-26. DOI: https://doi.org/10.1590/2317-1545v39n1166371.
(XI) Footitt, S., Douterelo-Soler, I., Clay, H., Finch-Savage, W.E. 2011. Dormancy cycling in Arabidopsis seeds is controlled by seasonally distinct hormone-signaling pathways. Proceedings of the National Academy of Science of the United States of America, 108(50), 20236-20241. DOI: https://doi.org/10.1073/pnas.1116325108.
(XII) França-Neto, J.B., Krzyzanowski, F.C. 2019. Tetrazolium: an important test for physiological seed quality evaluation. Journal of Seed Science, 41(3), 359-366. DOI: http://dx.doi.org/10.1590/2317-1545v41n3223104.
(XIII) Guedes, R.S., Alves, E.U., Gonçalves, E.P., Viana, J.S., Silva, K.B., Gomes, M.S.S. 2010. Metodologia para teste de tetrazólio em sementes de Amburana cearensis (Allemão) A.C. Smith. Revista Brasileira de Plantas Medicinais, 12(1), 120-126. DOI: https://doi.org/10.1590/S1516-05722010000100017.
(XIV) Hössel, C., Oliveira, J.S.M.A., Fabiane, K.C., Wagner Júnior, A., Citadin, I. 2013. Conservação e teste de tetrazólio em sementes de jabuticabeira. Revista Brasileira de Fruticultura, 35(1), 255-261. DOI: https://doi.org/10.1590/S0100-29452013000100029.
(XV) Huarte, H.R., Benech-Arnold, R.L. 2010. Hormonal nature of seed responses to fluctuating temperatures in Cynara cardunculus (L.). Seed Science Research, 20(1), 39-45. DOI: 10.1017/S0960258509990249.
(XVI) Jaganathan, G.K., Han, Y., Song, D., Selvam, P., Liu, B. 2019. Maternal and burial environment determine the physical dormancy release in tropical Senna auriculata (Fabaceae) seeds. Journal of Forestry Research, 30(4), 1343-1351. DOI: http://dx.doi.org/10.1007/s11676-018-0699-1.
(XVII) Kaiser, D.K., Freitas, L.C.N., Biron, R.P., Simonato, S.C., Bortolini, M.F. 2014. Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage. Journal of Seed Science, 36(3), 344-351. DOI: https://doi.org/10.1590/2317-1545v36n31022.
(XVIII) Labouriau, L.G., Pacheco, A. 1978. On the frequency of isothermal germination in seeds of Dolichos biflorus L. Plant and Cell Physiology, 19(3), 507-512. DOI: https://doi.org/10.1093/oxfordjournals.pcp.a075620.
(XIX) Labouriau, L.G., Valadares, M.E.B. 1976. On the germination of seeds of Calotropis procera (Ait.) Ait. F. Anais da Academia Brasileira de Ciências, 48(2), 263-284.
(XX) Lamarca, E.V., Barbedo, C.J. 2014. Methodology of the tetrazolium test for assessing the viability of seeds of Eugenia brasiliensis Lam., Eugenia uniflora L. and Eugenia pyriformis Cambess. Journal of Seed Science, 36(4), 427-434. DOI: http://dx.doi.org/10.1590/2317-1545v36n41029.
(XXI) Larcher, W. 2006. Ecofisiologia vegetal. São Carlos, RIMA Artes e Textos.
(XXII) Lopes, J.C., Capucho, M.T., Martins Filho, S., Repossi, P.A. 2005. Influência de temperatura, substrato e luz na germinação de sementes de bertalha. Revista brasileira de sementes, 27(2), 18-24. DOI: http://dx.doi.org/10.1590/S0101-31222005000200004.
(XXIII) Lorenzi, H. 2000. Árvores Brasileiras: Manual de Identificação e Cultivo de Plantas Arbóreas Nativas do Brasil, terceira ed. Nova Odessa, Plantarum.
(XXIV) Marcos-Filho, J. 2015. Fisiologia de sementes de plantas cultivadas. Londrina, ABRATES.
(XXV) Merai, Z., Graeber, K., Wilhelmsson, P., Ullrich, K.K., Arshad, W., Grosche, C., Tarkowská, D., Turečková, V., Strnad, M., Rensing, S.A., Leubner-Metzger, G., Scheid, O.M. 2019. Aethionema arabicum: a novel model plant to study the light control of seed germination. Journal of Experimental Botany, 70(12), 3313-3328. DOI: https://doi.org/10.1093/jxb/erz146.
(XXVI) Moore, R.P. 1972. Interpretation of color differences in tetrazolium testing. Seed Technologist News, 44(3), 22-24.
(XXVII) Naves, V.L., Rezende, R.M., Alcantra, E., Rezende, R.A.L.S. 2018. Superação de dormência de sementes de olho-de-cabra (Ormosia arborea) por diferentes métodos. Revista da Universidade do Vale do Rio Verde, 16(3), 1-8. DOI: http://dx.doi.org/10.5892/ruvrd.v16i3.5605.
(XXVIII) Nogueira, N.W., Torres, S.B., Freitas, R.M.O. 2014. Teste de tetrazólio em sementes de timbaúba. Semina: Ciências Agrárias, 35(6), 2967-2976. DOI: 10.5433/1679-0359.2014v35n6p2967.
(XXIX) Oliveira, A.K.M., Pereira, K.C.L. 2014. Efeito de diferentes temperaturas na germinação e crescimento radicular de sementes de jatobá-mirim (Guibourtia hymenaefolia (Moric.) J. Léonard). Ciência Florestal, 24(1), 111-116. DOI: https://doi.org/10.5902/1980509813328.
(XXX) Oliveira, A.K.M., Souza, J.S., Carvalho, J.M.B., Souza, S.A., Bocchese, R.A. 2016. Germinação de sementes e crescimento de Ormosia arborea em diferentes temperaturas e substratos. Gaia Scientia, 10(4), 262-271. DOI: http://dx.doi.org/10.21707/gs.v10.n04a21.
(XXXI) Pimenta, S.M., Coelho, M.F.B., Sales, D.M., Azevedo, R.A.B., Albuquerque, M.C.F. 2011. Germinação de sementes de Kielmeyera coriacea em diferentes substratos e condições de luz. Revista de Biologia e Ciências da Terra, 11(2), 81-87.
(XXXII) Reis, A.R.S., Santos, J.X., Carvalho, J.C., Leão, N.V.M., Freitas, A.D.D., Shimizu, E.C. 2016. Germinação, teste de tetrazólio, condutividade elétrica e embebição de sementes de amarelão (Apuleia moralis Spruce ex. Benth. – Fabaceae). Informativo ABRATES, 26(1,2,3), 36-47.
(XXXIII) Rosseto, J., Albuquerque, M.C.F., Rondon Neto, R.M., Silva, I.C.O. 2009. Germinação de sementes de Parkia pendula (Willd.) Benth. ex Walp. (Fabaceae) em diferentes temperaturas. Revista Árvore, 33(1), 47-55. DOI: https://doi.org/10.1590/S0100-67622009000100006.
(XXXIV) Sarmento, M.B., Silva, A.C.S., Villela, F.A., Santos, K.L., 2018. Biometria de frutos e sementes e crescimento pós-seminal de Acca sellowiana (O. Berg. Burret) Myrtaceae. Caderno de Pesquisa, 30 (1), 01-08. DOI: http://dx.doi.org/10.17058/cp.v30i1.8696.
(XXXV) Silva, A.L., Dias, D.C.F.S., Lima, L.B., Morais, G.A. 2014. Methods for overcoming seed dormancy in Ormosia arborea seeds, characterization and harvest time. Journal of Seed Science, 36(3), 318-325. DOI: https://doi.org/10.1590/2317-1545v36n31012.
(XXXVI) Silva, A.L., Morais, G.A. 2012. Biometry and dormancy breaking of Ormosia arborea seeds. Communications in Plant Sciences, 2(3-4), 105-107.
(XXXVII) Silva, R.C., Grzybowski, C.R.S., França-Neto, J.B., Panobianco, M. 2013. Adaptação do teste de tetrazólio para avaliação da viabilidade e vigor em sementes de girassol. Pesquisa Agropecuária Brasileira, 48(1), 105-113. DOI: https://doi.org/10.1590/S0100-204X2013000100014.
(XXXVIII) Souza, T.V., Voltolini, C.H., Santos, M., Paulilo, M.T.S. 2012. Water absorption and dormancy-breaking requirements of physically dormant seeds of Schizolobium parahyba (Fabaceae-Caesalpinioideae). Seed Science Research, 22(3), 169-176. DOI: https://doi.org/10.1017/S0960258512000013.
(XXXIX) Souza Filho, J.C., Coelho, M.F.B., Azevedo, R.A.B., Albuquerque, M.C.F. 2012. Germinação de sementes de Magonia pubescens St. Hil. - Sapindaceae em diferentes condições de luz e fotoperíodo. Revista de Biologia e Ciências da Terra, 12(2), 14-19.
(XL) Teixeira, W.F., Fagan, E.B., Casaroli, D., Canedo, S.C., Barbosa, K.A. 2011. Avaliação de métodos para superação de dormência na germinação de Ormosia arborea (Vell.) Harms. Biotemas, 24(4), 25-29. DOI: https://doi.org/10.5007/2175-7925.2011v24n4p25.
(XLI) Valadares, J., Paula, R.C. 2008. Temperaturas para germinação de sementes de Poecilanthe parviflora Bentham (Fabaceae-Faboideae). Revista Brasileira de Sementes, 30(2), 164-170. DOI: https://doi.org/10.1590/S0101-31222008000200020.
Downloads
Published
How to Cite
Issue
Section
License
The authors retain the rights to the manuscripts and, therefore, are free to share, copy, distribute, perform and publicly communicate the work under the following conditions:
Acknowledge work credits in the manner specified by the author or licensor (but not in a way that suggests that you have their support or that they support their use of their work).
REVISTA DE AGRICULTURA NEOTROPICAL (ISSN 2358-6303) is under license https://creativecommons.org/licenses/by/4.0/
The State University of Mato Grosso do Sul, Sustainable Development Center of Bolsão Sul-Mato-grossense (CEDESU), of the University Unit of Cassilândia (UUC), preserves the patrimonial rights (copyright) of the published works and favors and allows their reuse under the license as mentioned above.
------------
The journal reserves the right to make normative, orthographic, and grammatical alterations in the originals, to maintain the cult standard of the language, respecting, however, the style of the authors.
Final proofs will be sent to the authors.
Published works become the property of the journal. The opinions expressed by the authors of the manuscripts are their sole responsibility.