APPLICATION OF Azospirillum brasilense AND NITROGEN IN TOPDRESSING IN CORN HYBRIDS
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https://doi.org/10.32404/rean.v10i1.7055Keywords:
Diazotrophic bacteria, Growth promoting bacteria, Zea mays L.Abstract
Corn (Zea mays L.) is the most cultivated cereal in the world. The high yields obtained for this crop are mainly linked to nitrogen requirements. Technologies that aim to reduce costs with nitrogen fertilizers, with less environmental impact, such as diazotrophic bacteria, should be considered an advantageous alternative for this crop. The present study aimed to evaluate two corn hybrids, BioGene 7046® and Dekalb 310®, with three topdressing nitrogen doses (0.35 and 70 kg ha-1 of N) associated with four application methods of Azospirillum brasilense (without inoculation, seed inoculation, foliar inoculation, and the combination of inoculation via seeds and leaves). A randomized block design with four replications arranged in a 2x3x4 factorial scheme was used to evaluate variables related to growth and development, physiological analysis of nitrate reductase enzyme activity, and yield variables and their components. The use of Azospirillum brasilense did not affect the development of corn plants and did not increase grain yield. In the edaphoclimatic conditions in which this research was developed, nitrogen fertilization at doses of 35 and 70 kg ha-1 benefit nutrition, development, and corn yield components but are insufficient to increase grain yield.
References
(I) ANDA. ASSOCIAÇÃO NACIONAL PARA DIFUSÃO DE ADUBOS, 2021. Anuário estatístico do setor de fertilizantes. http://anda.org.br (accessed February 10, 2022).
(II) Baldani, V.L.D., Alvares, M.A.B., Baldani, J.I., Döbereiner, J. 1986. Establishment of inoculated Azospirillum spp. in the rhizosphere and roots of field grown wheat and sorghum. Plant and Soil, 90(1), 35-46. DOI: https://doi.org/10.1007/BF02277385
(III) Bashan, Y., Levanony, H. 1990. Current status of Azospirillum inoculation technology: Azospirillum as a challenge for agriculture. Canada Journal of Microbiology, 36(9), 593-596. DOI: https://doi.org/10.1139/m90-105
(IV) Bicudo, S.J., Cruz, S.C.S., Pereira, F.R.S., Brachtvogel, E.L., Madalena, J.A.S. 2009. Níveis de adubação para a cultura do milho safrinha. Revista Caatinga, 22(2), 23-30. https://periodicos.ufersa.edu.br/caatinga/article/view/1120/pdf
(V) Braccini, A.L., Dan, L.G.M., Piccinin, G.G., Albrecht, L.P., Barbosa, M.C., Ortiz, A.H. 2012. Seed inoculation with Azospirillum brasilense, associated with the use of bio regulators in maize. Revista Caatinga, 25(2) 58-64. https://periodicos.ufersa.edu.br/caatinga/article/view/2241
(VI) BRASIL. 2009. Regras para análise de sementes, Ministério da Agricultura, Pecuária e Abastecimento. 1ª ed. Secretaria de Defesa Sanitária, Brasília. https://www.gov.br/agricultura/pt-br/assuntos/insumos-agropecuarios/arquivos-publicacoes-insumos/2946_regras_analise__sementes.pdf (accessed May 14, 2014).
(VII) Bulla, D., Balbinot Junior, A.A. 2012. Inoculação de sementes de milho com Azospirillum brasiliense em diferentes doses de nitrogênio. Agropecuária Catarinense, 25(2), 61-63. https://publicacoes.epagri.sc.gov.br/RAC/article/view/652
(VIII) Borém, A., Galvão, J.C.C., Pimentel, M.A. 2017. Milho: do plantio à colheita. 2ª ed. Universidade Federal de Viçosa, Viçosa.
(IX) Coelho, A.M. 2010. Nutrição e adubação do milho. Embrapa milho e sorgo, Sete Lagoas. http://www.cnpsms.embrapa.br/publicacoes/milho_6_ed/ferraduba.htm (accessed March 8, 2014).
(X) Cruz, P.J., Carvalho, F.I.F., Silva, S.A., Kurek, A.J., Barbieri, R.L., Cargnin, A. 2003. Influência do acamamento sobre o rendimento de grãos e outros caracteres em trigo. Revista Brasileira de Agrociência, 9(1), 05-08. https://periodicos.ufpel.edu.br/ojs2/index.php/CAST/article/view/494/481
(XI) Cruz, S.C.S., Pereira, F.R.S., Santos, J.R., Albuquerque, A.W., Pereira, R.G. 2008. Adubação nitrogenada para o milho cultivado em sistema plantio direto, no Estado de Alagoas. Revista Brasileira de Engenharia Agrícola e Ambiental, 12(1), 62-68. DOI: https://doi.org/10.1590/S1415-43662008000100009
(XII) Dartora, J., Guimarães, V.F., Marini, D., Sander, G. 2013. Adubação nitrogenada associada à inoculação com Azospirillum brasilense e Herbaspirillum seropedicae na cultura do milho. Revista Brasileira de Engenharia Agrícola e Ambiental, 17(10), 1023-1029. DOI: https://doi.org/10.1590/S1415-43662013001000001
(XIII) Ferreira, D.F. 2011. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, 35(6), 1039-1042. DOI: https://doi.org/10.1590/S1413-70542011000600001
(XIV) García de Salamone, E., Dobereiner, J. 1996. Maize genotype effects on the response to Azospirillum inoculation. Biology and Fertility of Soils, 21, 193-196. DOI: https://doi.org/10.1007/BF00335934
(XV) Hungria, M. 2011. Inoculação com Azospirillum brasilense: inovação e rendimento a baixo custo. Embrapa Soja, Londrina. https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29676/1/Inoculacao-com-azospirillum.pdf (accessed July 5, 2021).
(XVI) Hungria, M., Campo, R.J., Souza, E.M., Pedrosa, F.O. 2010. Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant and Soil, 331(2), 413-425. DOI: https://doi.org/10.1007/s11104-009-0262-0
(XVII) Jaworski, E.G. 1971. Nitrate reductase assay in intact plant tissues. Biochemical and Biophysical Research Communications, 43(6), 1274-1279. https://www.sciencedirect.com/science/article/pii/S0006291X71800104
(XVIII) Kappes, C., Silva, R.G., Ferreira, V.E.N. 2017. Aplicação foliar de Azospirillum brasilense e doses de nitrogênio em cobertura no milho safrinha. Scientia Agraria Paranaensis, 16(3), 366-373. DOI: https//doi.org/10.18188/1983-1471/sap.v16n3p366-373
(XIX) Melero, M.M., Gitti, D.C., Arf, O., Rodrigues, R.A.F. 2013. Coberturas vegetais e doses de nitrogênio em trigo sob sistema plantio direto. Pesquisa Agropecuária Tropical, 43(4), 343-353. DOI: https://doi.org/10.1590/S1983-40632013000400001
XX. Meguro, N.E., Magalhães, A.C. 1982. Atividade da redutase de nitrato em cultivares de café. Pesquisa Agropecuária Brasileira, 17(2),1725-1731.
XXI. Oliveira, M.A., Zucareli, C., Spolaor, L.T., Domingues, A.R., Ferreira, A.S. 2012. Desempenho agronômico do milho sob adubação mineral e inoculação das sementes com rizobactérias. Revista Brasileira de Engenharia Agrícola e Ambiental, 16(10), 1040-1046. DOI: https://doi.org/10.1590/S1415-43662012001000002
XXII. Pandolfo, C.M., Vogt, G.A., Balbinot Junior, A.A., Gallotti, G.J.M., Zoldan, S.R. 2015. Desempenho de milho inoculado com Azospirillum brasilense associado a doses de nitrogênio em cobertura. Agropecuária Catarinense, 27(3), 94-99. https://publicacoes.epagri.sc.gov.br/RAC/article/view/567
XXIII. Prado, M.R., Campos, C.N.S. 2018. Nutrição e adubação de grandes culturas. 1ª ed. FCAV, Jaboticabal.
XXIV. Quadros, P.D., Roesch, L.F.W., Silva, P.R.F., Vieira, V.M., Roehrs, D.D., Camargo, F.A.O. 2014. Desempenho agronômico a campo de híbridos de milho inoculados com Azospirillum. Revista Ceres, 61(2), 209-218. DOI: http://dx.doi.org/10.1590/S0034-737X2014000200008
XXV. Riva Neto, D.C.O., Barros, L.R., Sousa, V.S., Brasil, E.P.F., Ribon, A.A, Smaniotto, A.O. 2021. Initial development of corn subjected to increasing nitrogen doses. Revista de Agricultura Neotropical, 8(1), 2-6. DOI: https://doi.org/10.32404/rean.v8i1.5226
XXVI. Souza, D.M.G., Lobato, E. 2004. Cerrado: correção do solo e adubação. 2ª ed. Brasília, Embrapa Cerrados. https://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/555355 (accessed January 10, 2022)
Taiz, L., Zeiger, E., Moller, I.M, Murphy, A. 2017. Plant Physiology and Development, sixth ed. Sinauer Associates, Sunderland.
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