REACTION OF Coffea arabica SEEDLINGS TO COLONIZATION BY WILD Colletotrichum gloeosporioides AND TRANSFORMED WITH gfp.
Visualizações: 66DOI:
https://doi.org/10.32404/rean.v11i4.8365Keywords:
Coffea arabica, Enzymes, ProteinsAbstract
Defense reactions of coffee trees in response to invasion by Colletotrichum gloeosporioides (Penz.) wild (I2) and transformed with the green fluorescent protein gene gfp (I2-T) were studied in this research to identify defense reactions of coffee tree seedlings in response to invasion by Colletotrichum gloeosporioides using wild isolates and those transformed with the gfp gene by observing the biochemical changes developed by the plants and to check the difference in the pathogen behavior after transformation. The activity of the guaiacol peroxidase (POX) enzyme was evaluated in both types of seedlings obtained, with and without symptoms (MOPCS and MOPSS), respectively. The inoculation of the different isolates (I2 and I2-T) induced the activity of the enzyme, showing higher rates than those observed in the control treatment, demonstrating that there was a possible recognition of the pathogen. Regarding the activity of the enzyme polyphenoloxidase (PPO), similar behavior was observed between the control and the isolates studied at different exposure times. The highest peak in the activity of the enzymes studied occurred at 6 and 24 hours after inoculation with I2. It was observed that the time the plants were exposed to the pathogen increased the levels of total soluble phenols.
References
(I) Armesto, C., Martins-Maia, F.G., Abreu, M.S., Figueira, A.R., Silva, B.M., Monteiro, F.P., 2012. Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot. Brazilian Journal of Microbiology, 43(3), 1222-1229. DOI: https://doi.org/10.1590/S1517-83822012000300050
(II) Anterola, A.M., Lewis, N.G., 2002. Trends in lignin modification: a comprehensive analysis of the effects of genetic manipulations/mutations on lignification and vascular integrity. Phytochemistry, Oxford, 61(3), 221-294. DOI: https://doi.org/10.1016/s0031-9422(02)00211-x
(III) Bar-on, Y.M., Phillips, R., Milo, R., 2018. The biomass distribution on Earth. Proceedings of the National Academy of Sciences of the United States of America, 115(25), 6506-6511. https://www.pnas.org/doi/full/10.1073/pnas.1711842115
(IV) Fernandes, C.D., Vieira Junior, J.R., Silva, D.S.G., Reis, N.D., Antunes Junior, H., 2009. Mecanismos de defesa de plantas contra o ataque de agentes fitopatogênicos. Porto Velho, RO: Embrapa Rondônia, 14 p.
(V) Chalfie, M., Euskirchen G., Prasher, D.C., 1994. Proteína Verde Fluorescente como Marcador de Expressão Gênica. Science, 263(5148), 802-805. DOI: https://doi.org/10.1126/science.
(VI) Dallagnol, L.J., Pazdiora, P.C., Dorneles, K.R., Morello, T.N., Nicholson P., 2021. Silicon soil amendment as a complement to manage tan spot and fusarium head blight in wheat. Agronomy for Sustainable Development, 41(21), 1-2. https://link.springer.com/article/10.1007/s13593-021-00677-0
(VII) Dong, O.X, Ronald, P.C., 2019. Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future Perspectives. Plant Physiology, 180(1), 26-38. DOI: https://doi.org/10.1104/pp.18.01224
(VIII) Evans, I., Solberg, E., Huber, D.M., 2009. Copper and plant disease. Mineral nutrition and plant disease. Saint Paul, MN. The American Phytopathological Society, 44(5), 177-188. DOI: https://doi.org/10.21273/HORTSCI.44.5.1510a
(IX) Ferreira, D.F., 2011. SISVAR: a computer statistical analysis system. Ciência e Agrotecnologia, Lavras, 35(6), 1039-1042. DOI: https://doi.org/10.1590/S1413-70542011000600001
(X) Kristensen, B.K., Bloch, H., Rasmussen, S.K., 1999. Barley coleoptiles peroxidases: purification, molecular cloning, and induction by pathogens. Plant Physiology, 120(2), 501-512. DOI: https://doi.org/10.1104%2Fpp.120.2.501
(XI) Loon, L.C., Van, Bakker, P.A.H.M., Pieterse, C.M.J., 1998. Systemic resistence induced by rhizosphere bactéria. Annual Review of Phytopathology, Palo Alto, 36(1), 453-483. DOI: https://doi.org/10.1146/annurev.phyto.36.1.453
(XII) Munaut, F., Hamaide, N., Vander Stappen, J., Maraite, H., 1998. Genetic relationships among isolates of Colletotrichum gloeosporioides from Stylosanthes spp. in Africa and Australia using RAPD and ribosomal DNA markers. Plant Pathology, 47(5), 641–648. DOI: https://doi.org/10.1046/j.1365-3059.1998.00287.x
(XIII) Nicholson, R.L., Hammerschmid T.R., 1992. Phenolic compounds and their role in disease resistance. Annual Review of Phytopathology, 30(8), 369-389. DOI: https://doi.org/10.1146/annurev.py.30.090192.002101.
(XIV) Paradela Filho, O., Paradela, A. L., 2001. O complexo Colletotrichum: cafeeiro. In: ZAMBOLIM, L. (Ed.). Tecnologias de produção de café com qualidade. Viçosa: UFV, Departamento de Fitopatologia, cap. 8. p. 269-275. DOI: https://doi.org/10.1590/S0006-87052005000300013
(XV) Pimenta, B.K., Vilela, E.V., Júnior, C.C., 2004. Componentes de parede celular de grãos de frutos de café (Coffea arabica L.) submetidos a diferentes tempos à espera da secagem. Acta Scientiarum. Agronomy, 26(2), 203-209. DOI: https://doi.org/10.4025/actasciagron.v26i2.1884
(XVI) Ribeiro Júnior, P.M., Resende, M.L.V., Pereira, R.B., Cavalcanti, F.B., Amaral, D.R., Pádua, M.A., 2006. Fosfito de potássio na indução de resistência a Verticillium dahliae Kleb., em mudas de cacaueiro (Theobroma cacao L.). Ciência Agrotecnologia, 30(4), 629-636. DOI: https://doi.org/10.1590/S1413-70542006000400006
(XVII) Salgado, P.R., Favarin, J.L., Leandro, R.A., Lima Filho, O.F., 2008. Total phenol concentration in coffee tree leaves during fruit development. Scientia Agricola, 65(4), 354-359. DOI: https://doi.org/10.1590/S0103-90162008000400005
(XVIII) Shiraishi, T., Yamada, T., Nicholson, R.L., Kunoh, H., 1995. Phenylalanine ammonia-lyase in barley: activity enhancement in response to Erysiphe graminis f. sp. hordei (race 1) a pathogen, and Erysiphe pisi, a nonpathogen. Physiological and Molecular Plant Pathology, 46(2), 153-162. DOI: https://doi.org/10.1006/pmpp.1995.1012
(XIX) Stangarlin, J.R., Kuhn, O.J., Toledo, M.V., Portz, R.L., Schwan-Estrada, K.R.F., Pascholati, S.F., 2011. A defesa vegetal contra fitopatógenos. Scientia Agraria Paranaenis, 10(1), 18-46. https://e-revista.unioeste.br/index.php/scientiaagraria/article/view/5268
(XX) Vieira, J.F., Abreu, M.S., Maia, F.G.M., Ogoshi, C., Pierre, R.O., Carvalho, E.A., Silva, B.M., 2011. Tratamento térmico de sementes no manejo da mancha manteigosa causada por Colletotrichum gloeosporioides em cafeeiro Coffea arabica L. Acta Ambiental Catarinense, 8(1/2), 28-38. https://bell.unochapeco.edu.br/revistas/index.php/acta/article/view/1927
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors retain the rights to the manuscripts and, therefore, are free to share, copy, distribute, perform and publicly communicate the work under the following conditions:
Acknowledge work credits in the manner specified by the author or licensor (but not in a way that suggests that you have their support or that they support their use of their work).
REVISTA DE AGRICULTURA NEOTROPICAL (ISSN 2358-6303) is under license https://creativecommons.org/licenses/by/4.0/
The State University of Mato Grosso do Sul, Sustainable Development Center of Bolsão Sul-Mato-grossense (CEDESU), of the University Unit of Cassilândia (UUC), preserves the patrimonial rights (copyright) of the published works and favors and allows their reuse under the license as mentioned above.
------------
The journal reserves the right to make normative, orthographic, and grammatical alterations in the originals, to maintain the cult standard of the language, respecting, however, the style of the authors.
Final proofs will be sent to the authors.
Published works become the property of the journal. The opinions expressed by the authors of the manuscripts are their sole responsibility.