REACTION OF Coffea arabica SEEDLINGS TO COLONIZATION BY WILD Colletotrichum gloeosporioides AND TRANSFORMED WITH gfp.

Visualizações: 66

Authors

DOI:

https://doi.org/10.32404/rean.v11i4.8365

Keywords:

Coffea arabica, Enzymes, Proteins

Abstract

Defense reactions of coffee trees in response to invasion by Colletotrichum gloeosporioides (Penz.) wild (I2) and transformed with the green fluorescent protein gene gfp (I2-T) were studied in this research to identify defense reactions of coffee tree seedlings in response to invasion by Colletotrichum gloeosporioides using wild isolates and those transformed with the gfp gene by observing the biochemical changes developed by the plants and to check the difference in the pathogen behavior after transformation. The activity of the guaiacol peroxidase (POX) enzyme was evaluated in both types of seedlings obtained, with and without symptoms (MOPCS and MOPSS), respectively. The inoculation of the different isolates (I2 and I2-T) induced the activity of the enzyme, showing higher rates than those observed in the control treatment, demonstrating that there was a possible recognition of the pathogen. Regarding the activity of the enzyme polyphenoloxidase (PPO), similar behavior was observed between the control and the isolates studied at different exposure times. The highest peak in the activity of the enzymes studied occurred at 6 and 24 hours after inoculation with I2. It was observed that the time the plants were exposed to the pathogen increased the levels of total soluble phenols.

Author Biographies

Fernanda Gonçalves Martins Maia, Universidade Federal do Triângulo Mineiro

Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brasil.

Maísa de Paula Freitas, Universidade Federal do Triângulo Mineiro

Universidade Federal do Triângulo Mineiro, Campus Iturama, Iturama, Minas Gerais, Brasil. 

Jader Braga Maia, Universidade Federal do Triângulo Mineiro

Universidade Federal do Triângulo Mineiro, Campus Iturama, Iturama, Minas Gerais, Brasil.

Cecília Armesto, Unisepe Educacional / UniVR - Centro Universitário do Vale do Ribeira

Centro Universitário do Vale do Ribeira, Registro, São Paulo, Brasil. 

Pablo da Costa Gontijo, Instituto Federal Goiano

Instituto Federal Goiano, Campus Rio Verde, Rio Verde, Goiás, Brasil.

References

(I) Armesto, C., Martins-Maia, F.G., Abreu, M.S., Figueira, A.R., Silva, B.M., Monteiro, F.P., 2012. Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot. Brazilian Journal of Microbiology, 43(3), 1222-1229. DOI: https://doi.org/10.1590/S1517-83822012000300050

(II) Anterola, A.M., Lewis, N.G., 2002. Trends in lignin modification: a comprehensive analysis of the effects of genetic manipulations/mutations on lignification and vascular integrity. Phytochemistry, Oxford, 61(3), 221-294. DOI: https://doi.org/10.1016/s0031-9422(02)00211-x

(III) Bar-on, Y.M., Phillips, R., Milo, R., 2018. The biomass distribution on Earth. Proceedings of the National Academy of Sciences of the United States of America, 115(25), 6506-6511. https://www.pnas.org/doi/full/10.1073/pnas.1711842115

(IV) Fernandes, C.D., Vieira Junior, J.R., Silva, D.S.G., Reis, N.D., Antunes Junior, H., 2009. Mecanismos de defesa de plantas contra o ataque de agentes fitopatogênicos. Porto Velho, RO: Embrapa Rondônia, 14 p.

(V) Chalfie, M., Euskirchen G., Prasher, D.C., 1994. Proteína Verde Fluorescente como Marcador de Expressão Gênica. Science, 263(5148), 802-805. DOI: https://doi.org/10.1126/science.

(VI) Dallagnol, L.J., Pazdiora, P.C., Dorneles, K.R., Morello, T.N., Nicholson P., 2021. Silicon soil amendment as a complement to manage tan spot and fusarium head blight in wheat. Agronomy for Sustainable Development, 41(21), 1-2. https://link.springer.com/article/10.1007/s13593-021-00677-0

(VII) Dong, O.X, Ronald, P.C., 2019. Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future Perspectives. Plant Physiology, 180(1), 26-38. DOI: https://doi.org/10.1104/pp.18.01224

(VIII) Evans, I., Solberg, E., Huber, D.M., 2009. Copper and plant disease. Mineral nutrition and plant disease. Saint Paul, MN. The American Phytopathological Society, 44(5), 177-188. DOI: https://doi.org/10.21273/HORTSCI.44.5.1510a

(IX) Ferreira, D.F., 2011. SISVAR: a computer statistical analysis system. Ciência e Agrotecnologia, Lavras, 35(6), 1039-1042. DOI: https://doi.org/10.1590/S1413-70542011000600001

(X) Kristensen, B.K., Bloch, H., Rasmussen, S.K., 1999. Barley coleoptiles peroxidases: purification, molecular cloning, and induction by pathogens. Plant Physiology, 120(2), 501-512. DOI: https://doi.org/10.1104%2Fpp.120.2.501

(XI) Loon, L.C., Van, Bakker, P.A.H.M., Pieterse, C.M.J., 1998. Systemic resistence induced by rhizosphere bactéria. Annual Review of Phytopathology, Palo Alto, 36(1), 453-483. DOI: https://doi.org/10.1146/annurev.phyto.36.1.453

(XII) Munaut, F., Hamaide, N., Vander Stappen, J., Maraite, H., 1998. Genetic relationships among isolates of Colletotrichum gloeosporioides from Stylosanthes spp. in Africa and Australia using RAPD and ribosomal DNA markers. Plant Pathology, 47(5), 641–648. DOI: https://doi.org/10.1046/j.1365-3059.1998.00287.x

(XIII) Nicholson, R.L., Hammerschmid T.R., 1992. Phenolic compounds and their role in disease resistance. Annual Review of Phytopathology, 30(8), 369-389. DOI: https://doi.org/10.1146/annurev.py.30.090192.002101.

(XIV) Paradela Filho, O., Paradela, A. L., 2001. O complexo Colletotrichum: cafeeiro. In: ZAMBOLIM, L. (Ed.). Tecnologias de produção de café com qualidade. Viçosa: UFV, Departamento de Fitopatologia, cap. 8. p. 269-275. DOI: https://doi.org/10.1590/S0006-87052005000300013

(XV) Pimenta, B.K., Vilela, E.V., Júnior, C.C., 2004. Componentes de parede celular de grãos de frutos de café (Coffea arabica L.) submetidos a diferentes tempos à espera da secagem. Acta Scientiarum. Agronomy, 26(2), 203-209. DOI: https://doi.org/10.4025/actasciagron.v26i2.1884

(XVI) Ribeiro Júnior, P.M., Resende, M.L.V., Pereira, R.B., Cavalcanti, F.B., Amaral, D.R., Pádua, M.A., 2006. Fosfito de potássio na indução de resistência a Verticillium dahliae Kleb., em mudas de cacaueiro (Theobroma cacao L.). Ciência Agrotecnologia, 30(4), 629-636. DOI: https://doi.org/10.1590/S1413-70542006000400006

(XVII) Salgado, P.R., Favarin, J.L., Leandro, R.A., Lima Filho, O.F., 2008. Total phenol concentration in coffee tree leaves during fruit development. Scientia Agricola, 65(4), 354-359. DOI: https://doi.org/10.1590/S0103-90162008000400005

(XVIII) Shiraishi, T., Yamada, T., Nicholson, R.L., Kunoh, H., 1995. Phenylalanine ammonia-lyase in barley: activity enhancement in response to Erysiphe graminis f. sp. hordei (race 1) a pathogen, and Erysiphe pisi, a nonpathogen. Physiological and Molecular Plant Pathology, 46(2), 153-162. DOI: https://doi.org/10.1006/pmpp.1995.1012

(XIX) Stangarlin, J.R., Kuhn, O.J., Toledo, M.V., Portz, R.L., Schwan-Estrada, K.R.F., Pascholati, S.F., 2011. A defesa vegetal contra fitopatógenos. Scientia Agraria Paranaenis, 10(1), 18-46. https://e-revista.unioeste.br/index.php/scientiaagraria/article/view/5268

(XX) Vieira, J.F., Abreu, M.S., Maia, F.G.M., Ogoshi, C., Pierre, R.O., Carvalho, E.A., Silva, B.M., 2011. Tratamento térmico de sementes no manejo da mancha manteigosa causada por Colletotrichum gloeosporioides em cafeeiro Coffea arabica L. Acta Ambiental Catarinense, 8(1/2), 28-38. https://bell.unochapeco.edu.br/revistas/index.php/acta/article/view/1927

Downloads

Published

2024-11-29

How to Cite

Gonçalves Martins Maia, F., de Paula Freitas, M., Braga Maia, J., Armesto, C., & da Costa Gontijo, P. (2024). REACTION OF Coffea arabica SEEDLINGS TO COLONIZATION BY WILD Colletotrichum gloeosporioides AND TRANSFORMED WITH gfp. REVISTA DE AGRICULTURA NEOTROPICAL, 11(4). https://doi.org/10.32404/rean.v11i4.8365