CULTURE MEDIA EVALUATION ON THE Leucoagaricus gongylophorus AND Escovopsis sp. FUNGI DEVELOPMENT

Visualizações: 523

Authors

  • Maria Lucidalva Ribeiro de Sousa Universidade Federal do Amazonas. Instituto de Ciências Biológicas. Departamento de Morfologia. UFAM. Manaus
  • Janaína da Costa Nogueira Universidade Federal do Amazonas. Instituto de Ciências Biológicas. Departamento de Morfologia. UFAM. Manaus
  • Adriana Dantas Gonzaga Freitas Universidade Federal do Amazonas. Instituto de Ciências Biológicas. Departamento de Morfologia. UFAM. Manaus

DOI:

https://doi.org/10.32404/rean.v8i3.5869

Abstract

This study aimed to evaluate the in vitro interference of different culture media, Czapek Dox Agar, Nutrient Agar, Starch Casein agar, Malt Extract Broth Base, Nutrient Broth, and the medium containing Peptone, Malt, Agar and crushed peach palm pulp (Bactris gasipaes) and tucumã (Astrocaryum aculeatum) on the fungus Leucoagaricus gongylophorus and the parasitic fungus Escovopsis sp. growth. The fungi were inoculated in different culture media and then kept in acclimatized chambers at 28 ºC in the dark for 42 days, being analyzed at weekly intervals, totaling six evaluations. Fungal colonies were evaluated considering the factor "f" (cm), obtained from the product between the width (W) and length (L) of the diameter of the colonies (f = W x L). The results showed that the symbiotic fungus and the leafcutter ant parasitic fungus showed a growth increase compared to the media provided in the plates, and the media added with tucumã peel, tucumã peel+pulp, fresh peach palm, and cooked peach palm had a significant effect in the L. gongylophorus fungus development. However, these treatments were not promising for the Escovopsis sp. fungus compared to other culture media.

Author Biographies

Maria Lucidalva Ribeiro de Sousa, Universidade Federal do Amazonas. Instituto de Ciências Biológicas. Departamento de Morfologia. UFAM. Manaus

Graduanda em Ciências Naturais pela Universidade Federal do Amazonas, Av. General Rodrigo Otávio, 6200, Coroado 1, Cep: 69080-900, Manaus-Am, Brasil

Janaína da Costa Nogueira, Universidade Federal do Amazonas. Instituto de Ciências Biológicas. Departamento de Morfologia. UFAM. Manaus

Docente convidada pela Universidade Federal do Amazonas, Av. General Rodrigo Otávio, 6200, Coroado 1, Cep: 69080-900, Manaus-Am, Brasil.

Adriana Dantas Gonzaga Freitas, Universidade Federal do Amazonas. Instituto de Ciências Biológicas. Departamento de Morfologia. UFAM. Manaus

Docente da Universidade Federal do Amazonas – Instituto de Ciências Biológicas – ICB/UFAM, Departamento de Morfologia. Av. General Rodrigo Octávio, 6200, Coroado I, Cep: 69080-900, Manaus, Amazonas Brasil

References

(I) Azevedo, J.L.; Costa, S.O.P., 1973. Exercícios práticos de genética, São Paulo: EDUSP, 288 p.

(II) Borba, R.S.; Loecki, A.E.; Bandeira, J.M.; Moraes, C.L.; Centenaro. E.D., 2006. Crescimento do fungo simbionte de formigas cortadeiras do gênero Acromyrmex em meios de cultura com diferentes extratos. Ciência Rural, Santa Maria, 36(3),725-730. DOI: https://doi.org/10.1590/S0103-84782006000300002.

(III) Barbosa, F.H.F; Barbosa, L.P.J.L., 2010. Alternativas Metodológicas em Microbiologia: viabilizando atividades práticas. Revista de Biologia e Ciências da Terra, 10(2), 134-143. http://joaootavio.com.br/bioterra/workspace/uploads/artigos/artigo_15_v10_n2-51562daa0b616.pdf. (acessado 10 janeiro de 2020).

(IV) Camargo, R.S.; Forti, L.C.; Rocha, M.M.; Matos, C.A.O.; Lopes, J.F.; Andrade, A.P.P.; Verza, S.S., 2003. The effect of plant diversity on fungus garden development and foraging behavior of leaf-cutting ants (Hymenoptera: Formicidae)., 2003. Sociobiology, Chico, 42(2), 1-10. http://www.csuchico.edu/biol/Sociobiology/volume/sociobiologyv42n22003.html#11. (acessado 10 de janeiro de 2020).

(V) Cantarelli, E.B.; Costa, E.C.; Pezzutti, R.V.; Zanetti, R.; Fleck, M.D. 2019. Damage by Acromyrmex spp. to an initial Pinus taeda L. planting. Floresta e Ambiente, 26(4), 1-7. https://doi.org/10.1590/2179-8087.006016.

(VI) Currie, C.R.; Mueller, U.G.; Malloch, D., 1999a. The agricultural pathology of ant fungus gardens. Proceedings of the National Academy of Sciences, 96(14), 7998-8002. https://doi.org/10.1038/19519

(VII) Currie, C.R.; Scott, J.A.; Summerbell, R.C.; Malloch, D. 1999b. Fungus-growing ants use antibiotic-producing bacteria to control garden parasites. Nature, 398, 701-704. https://doi.org/10.1038 / 19519

(VIII) Castelani, A., 1939. Viability of mold culture of fungi in destilled water. J Trop Med & Hyg.

(IX) Demirtas, A.; Ozturk, H.; Piskin, I. 2018. Overview of plant extracts and plant secondary metabolites as alternatives to antibiotics for modification of ruminal fermentation. Ankara universitesi Veteriner Fakultesi Dergisi, 65(2)13-217. https://doi.org/10.1501/Vetfak_0000002849

(X) Della Lucia, T.M.C., Souza, D.J. Importância e história de vida das formigas cortadeiras. In: Della Lucia, T.M.C. (Ed.). Formigas cortadeiras – da Bioecologia ao Manejo, Viçosa, MG: Editora UFV, 2011. p. 13-26.

(XI) Della Lucia, T.M.C.; Gandra, L.C.; Guedes, R.N. 2014. Managing leaf‐cutting ants: peculiarities, trends and challenges. Pest management science, 70(1), 14-23. https://doi.org/10.1002/ps.3660.

(XII) Ferreira, D. F. 2014 Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e Agrotecnologia, Lavras, 38(2), 109-112. https://doi.org/10.1590/S1413-70542014000200001

(XIII) Garcia. L.F.M, Sagrilo. M.R.; Souza-Filho. O.C.; Ribeiro. E.E. 2013. The In Vitro Genotoxic Effect of Tucuma (Astrocaryum aculeatum), an Amazonian Fruit Rich in Carotenoids. Journal of Medicinal Food, 16 (2), 1013-1021. https://doi.org/10.1089/jmf.2012.0287

(XIV) Koch, A.L. 1975. The kinects of mycelial growth. Journal of General Microbiology, 89(2), 209-216. DOI: https://doi.org/10.1099/00221287-89-2-209

(XV) Loeck, A.E.; Pierobom, C.R.; Gusmão, L.G.; Afonso, A.P. 2004. Growth of symbiont fungi of some higher Attini ants in mineral médium. Ciência Rural, Santa Maria, 34(1), 79-82. DOI: https://doi.org/10.1590/S0103-84782004000100012

(XVI) Man, T.J.B.; Stajich, J.E.; Kubicekc, C.P.; Teiling, C.; Chenthamara, K.; Atanasova, L.; Druzhinina, I.S.; Levenkova, N.; Birnbaum, S.S.L.; Barribeau, S.M.; Bozick, B.A; Suen, G.; Currie, C.R.; Gerardo, N.M. 2016. Small genome of the fungus

Escovopsis weberi, a specialized disease agent of ant agriculture. Proceedings of the National Academy of Sciences of the United States of America, Washington, 113(13), 3567-3572. DOI: https://doi.org/10.1073/pnas.1518501113.

(XVII) Moreno, L.,F.; Feng, P.; Weiss, V.A.; Vicente, V.A.; Stielow, B.; Hoog, S. 2017. Phylogenomic analyses reveal the diversity of laccase-coding genes in Fonsecaea genomes. Plos one, 12(1), 171-291. DOI: https://doi.org/10.1371/journal.pone.0171291.

(XVIII) Mueller, U.G.; Kardish, M.R.; Ishak, H.D.; Wright, A.M.; Solomon, S.E.; Bruschi, S.M.; Carlson, A.L.; Bacci Jr, M. 2018. Phylogenetic patterns of ant–fungus associations indicate that farming strategies, not only a superior fungal cultivar, explain the ecological success of leafcutter ants. Molecular Ecology, 27(10), 2414–2434. DOI: https://doi.org/10.1111/mec.14588.

(XIX) Nepomucena, R.M.P. 2010. Avaliação do potencial microbiano do crescimento e de secreção de lacase do fungo amazônico lentinus crinitus (L. ex Fr.) (Dissertação de Mestrado) Biotecnologia e Recursos Naturais, Universidade Estadual do Amazonas, Manaus, AM. http://repositorioinstitucional.uea.edu.br//handle/riuea/2216. (acessado 10 de janeiro de 2020).

(XX) Pagnocca, F.C.; Rodrigues. A.; Jr. Bacci, M. 2011. Microrganismos associados as formigas-cortadeiras. In: Della lucia, T.M.C. (Ed.) Formigas-cortadeiras: da bioecologia ao manejo. Viçosa: Editora da UFV, 262-283.

(XXI) Reynolds, H.T.; Currie, C.R. 2004. Pathogenicity of Escovopsis weberi: The parasite of the attine ant-microbe symbiosis directly consumes the ant-cultivated fungus. Mycologia, Lawrence, 96(5), 955–959. PMID: 21148916.

(XXII) Seipke, R., Crossman, L., Drou, N., Darren, H., Mervyn, J., Caccamo, M.; Matthew, I. 2011. Draft genome sequence of Streptomyces Strain S4, a symbiont of the leafcutting ant Acromyrmex octospinosus. J Bacteriol, 193, 4270– 4271. https://doi.gor/10.1128 / jb.05275-11

(XXIII) Siqueira, C.G., Bacci, M., Pagnocca, F. C., Bueno, O. C., Hebling, M.J. 1998. Metabolism of plant polysaccharides by Leucoagaricus gongylophorus, the symbiotic fungus of the leafcutting ant Atta sexdens L. Applied and Environmental Microbiology, 64, 4820-4822. https://doi.org/10.1128/AEM.64.12.4820-4822.1998.

(XXIV) Scalbert, A. 1991. Antimicrobial properties of tannins. Phytochemistry, 30(12), 3875-3883. https://doi.org/10.1016/0031-9422(91)83426-L.

(XXV) Victor, S.R., Crisostomo, F.R., Bueno, F.C., Pagnocca, F.C., Fernandes, J.B., Correa, A.G.; Silva, M.F.G.F. 2001. Toxicity of synthetic piperonyl compounds to leaf-cutting ants and their symbiotic fungus. Pest Management Science, 57(7), 603-608. https://doi.org/10.1002/ps.333.

(XXVI) Zanetti, R.; Zanuncio, J.C.; Santos, J.C.; Silva, W.L.P.; Ribeiro, G.T.; Lemes, P.G. 2014. An overview of integrated management of leaf-cutting ants (Hymenoptera: Formicidae) in Brazilian forest plantations. Forests, 5(3), 439-454. https://doi.org/10.3390/f5030439

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Published

2021-08-31

How to Cite

Sousa, M. L. R. de, Nogueira, J. da C., & Freitas, A. D. G. (2021). CULTURE MEDIA EVALUATION ON THE Leucoagaricus gongylophorus AND Escovopsis sp. FUNGI DEVELOPMENT. REVISTA DE AGRICULTURA NEOTROPICAL, 8(3), e5869. https://doi.org/10.32404/rean.v8i3.5869

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Vol. 8 No. 3 (2021): REVISTA DE AGRICULTURA NEOTROPICAL

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